抗Human TNF-alpha抗体(Anti-Human TNF-alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human TNF-alpha Gly57-Leu233Accession # P01375
Source	Monoclonal Mouse IgG1 Clone # 1825
Purification	Protein A or G purified from ascites
Specificity	Detects human TNF-alpha in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant rhesus macaque TNF-alpha is observed and no cross-reactivity with recombinant cotton rat, mouse, rat, or porcine TNF-alpha is observed. It shows no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA‑A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhRANK Ligand, rhTNF-beta, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI.

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Applications and Data

	Recommended Concentration	Sample
Neutralization	Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.06 µg/mL in the presence of 0.75 ng/mL Recombinant Human TNF‑ alpha and 1 µg/mL actinomycin D.

Neutralization

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody. Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB210). The ND50 is typically 0.01‑0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

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Related Product & Information

Long Name	Tumor Necrosis Factor alpha
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Background	TNF-alpha
background_content	Background: TNF-alpha TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.

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Citations

1. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
   Authors: J Uebele, C Stein, MT Nguyen, A Schneider, F Kleinert, O Tichá, G Bierbaum, F Götz, I Bekeredjia
   PLoS Pathog., 2017;13(5):e1006387.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
   Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
   PLoS Pathog., 2017;13(9):e1006577.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Activin A as a mediator of NK-dendritic cell functional interactions.
   Authors: Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
   J Immunol, 2014;192(3):1241-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
   Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
   PLoS Biol, 2012;10(9):e1001395.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
   Authors: Nicolaidou V, Wong MM, Redpath AN
   PLoS ONE, 2012;7(7):e39871.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
   Authors: Navenot JM, Fujii N, Peiper SC
   Mol. Pharmacol., 2009;75(5):1074-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
   Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
   Dig. Dis. Sci., 2007;52(10):2775-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
   Authors: Arnold R, Neumann M, Konig W
   Immunology, 2007;121(1):71-81.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
   Authors: Ebert EC, Mehta V
   Cell. Immunol., 2006;241(1):7-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
    Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
    Exp. Hematol., 2006;34(10):1312-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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