抗Human IL-4抗体(Anti-Human IL-4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑4His25-Ser153Accession # P05112
Source	Monoclonal Mouse IgG2B Clone # 34019
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse IL‑4, recombinant porcine IL‑4, recombinant cotton rat IL‑4, recombinant rat IL‑4, or recombinant human IL‑13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑4 (Catalog # 204-IL)
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Neutralization

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB204). The ND50 is typically 0.5-1.5 µg/mL.

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Related Product & Information

Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Background	IL-4
background_content	Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

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Citations

1. Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.
   Authors: Kongsbak M, von Essen M, Boding L, Levring T, Schjerling P, Lauritsen J, Woetmann A, Odum N, Bonefeld C, Geisler C
   PLoS ONE, 2014;9(5):e96695.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Impaired development of human Th1 cells in patients with deficient expression of STAT4.
   Authors: Chang HC, Han L, Goswami R, Nguyen ET, Pelloso D, Robertson MJ, Kaplan MH
   Blood, 2009;113(23):5887-90.
   Species: Human
   Sample Type: Whole Cells
   Application: Other: Cell differentiation


3. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
   Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
   J. Exp. Med., 2009;206(4):793-805.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


4. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
   Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
   Nat. Immunol., 2007;8(9):942-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Cytokine milieu of atopic dermatitis skin subverts the innate immune response to vaccinia virus.
   Authors: Howell MD, Gallo RL, Boguniewicz M, Jones JF, Wong C, Streib JE, Leung DY
   Immunity, 2006;24(3):341-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
   Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
   Clin. Immunol., 2006;120(2):220-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
   Authors: Ebert EC, Mehta V
   Cell. Immunol., 2006;241(1):7-13.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Cutting edge: immune cells as sources and targets of the IL-10 family members?
   Authors: Wolk K, Kunz S, Asadullah K, Sabat R
   J. Immunol., 2002;168(11):5397-402.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay

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