抗Human IL-2抗体(Anti-Human IL-2 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑2Ala21-Thr153Accession # NP_000577
Source	Monoclonal Mouse IgG1 Clone # 5334
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.

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Applications and Data

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde, and permeabilized with saponin
Neutralization	Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.

Neutralization
	Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.
Immunocytochemistry
	IL‑2 in Human PBMCs. IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Background	IL-2
background_content	Background: IL-2 Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

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Citations

1. IL-23 and IL-1? Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation
   Authors: S Revu, J Wu, M Henkel, N Rittenhous, A Menk, GM Delgoffe, AC Poholek, MJ McGeachy
   Cell Rep, 2018;22(10):2642-2653.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells
   Authors: T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


3. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
   Sci Rep, 2016;6(0):32320.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Activin A programs the differentiation of human TFH cells
   Nat Immunol, 2016;17(8):976-84.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


5. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
   Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
   J Biol Chem, 2015;290(37):22338-51.
   Species: Human
   Sample Type: Protein
   Application: Neut


6. Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells.
   Authors: Kim, Dae Won, Zloza, Andrew, Broucek, Joseph, Schenkel, Jason M, Ruby, Carl, Samaha, Georges, Kaufman, Howard L
   J Transl Med, 2014;12(0):113.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
   Blood, 2012;119(26):6268-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
   Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
   Vaccine, 2010;28(8):1932-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
   Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
   Blood, 2007;109(11):4856-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue.
    Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A
    Cancer Res., 2005;65(18):8101-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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