抗Human/Mouse/Rat CCR5 Allophycocyanin抗体(Anti-Human/Mouse/Rat CCR5 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Allophycocyanin
Immunogen	CHO Chinese hamster ovary cell line transfected with human CCR5
Source	Monoclonal Mouse IgG1 Clone # CTC5
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL‑12 (Catalog # 419‑ML) and 10 μg/mL Goat Anti-Mouse IL‑4 Poly­clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background	CCR5
background_content	Background: CCR5 CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

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Citations

1. CCR5 antagonist blocks metastasis of basal breast cancer cells.
   Authors: Velasco-Velazquez M, Jiao X, De la Fuente M, Pestell T, Ertel A, Lisanti M, Pestell R
   Cancer Res, 2102;72(15):3839-50.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. CCR5 governs DNA damage and breast cancer stem cell expansion
   Authors: X Jiao, MA Velasco-Ve, M Wang, Z Li, H Rui, AR Peck, JE Korkola, X Chen, S Xu, JB DuHadaway, S Guerrero-R, S Addya, D Sicoli, Z Mu, G Zhang, A Stucky, X Zhang, M Cristofani, A Fatatis, JW Gray, JF Zhong, GC Prendergas, RG Pestell
   Cancer Res., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. CIB1 and CIB2 are HIV-1 helper factors involved in viral entry
   Sci Rep, 2016;6(0):30927.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CCR5 receptor antagonists block metastasis to bone of v-Src oncogene-transformed metastatic prostate cancer cell lines.
   Authors: Sicoli D, Jiao X, Ju X, Velasco-Velazquez M, Ertel A, Addya S, Li Z, Ando S, Fatatis A, Paudyal B, Cristofanilli M, Thakur M, Lisanti M, Pestell R
   Cancer Res, 2015;74(23):7103-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
   Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
   J Immunol, 2014;192(6):2634-42.
   


6. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
   Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
   AIDS, 2010;24(4):491-502.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
   Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
   J. Immunol., 2007;178(7):4184-93.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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