抗GDF-15抗体(Anti-GDF-15, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27984
GDF-15に対する抗体(Anti-GDF-15, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年06月08日 00時01分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Anti-GDF-15, Human, Goat-Poly, Biotin |
|
7 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年06月08日 00時01分現在]
Anti-GDF-15, Human, Goat-Poly, Biotin
文献数： 7
- 商品コード：BAF940
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human GDF-15 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB957-500，スタンダードとして#957-GD-025を用いる。 別名：GDF15 Genbank No： 9518 Protein Accession No： Q99988 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
追加しました。
Product Details
| Species Reactivity | Human, Primate |
|---|---|
| Label | Biotin |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human GDF-15 (R&D Systems, Catalog # 957-GD)Ala197-Ile308Accession # Q99988 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human GDF-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, and rmGDF-8 is observed. |
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human GDF‑15 (Catalog # 957-GD) |
| Human/Primate GDF-15 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate GDF‑15 Antibody (Catalog #MAB957 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human/Primate GDF‑15 Biotinylated Antibody (Catalog #BAF940 ) |
| ELISA Standard | Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog #957-GD ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: GDF-15 | Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival. |
|
追加しました。
References
| Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514. | |
| Böttner, M. et al. (1999) Gene 237:105. | |
| Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2. | |
| Fairlie, W.D. et al. (2001) J B.C 20:16911. | |
| Bauskin, A.R. et al. (2000) EMBO J. 19:2212. | |
| Strelau, J. et al. (2000) J. Neurosci. 20:8597. | |
| Schober, A. et al. (2001) J. Comp. Neurol. 439:32. | |
| Long Name: | Growth Differentiation Factor 15 |
| Entrez Gene IDs: | 9518 (Human); 23886 (Mouse); 29455 (Rat) |
| Alternate Names: | GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta |
追加しました。
Related Research Areas
| BMP Family | ||
| Growth/Differentiation Factors (GDFs) | ||
| TGF-beta Superfamily | ||
| ||
追加しました。
Related Product & Information
| Background | GDF-15 |
|---|---|
| background_content | Background: GDF-15 Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival. |
追加しました。
Citations
- Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
Authors: Li, Ji, Veltri, Robert W, Yuan, Zhen, Christudass, Christhu, Mandecki, Wlodek
PLoS ONE, 2015;10(4):e0122249.
Species: Human
Sample Type: Serum
Application: ELISA detection - AF1q: A Novel Mediator of Basal and 4-HPR-Induced Apoptosis in Ovarian Cancer Cells.
Authors: Tiberio P, Cavadini E, Callari M
PLoS ONE, 2012;7(6):e39968.
Species: Human
Sample Type: Cell Lysates
Application: WB - Growth differentiation factor-15 as a prognostic marker in patients with acute myocardial infarction.
Authors: Khan SQ, Ng K, Dhillon O, Kelly D, Quinn P, Squire IB, Davies JE, Ng LL
Eur. Heart J., 2009;30(9):1057-65.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Growth differentiation factor-15 predicts mortality and morbidity after cardiac resynchronization therapy.
Authors: Foley PW, Stegemann B, Ng K, Ramachandran S, Proudler A, Frenneaux MP, Ng LL, Leyva F
Eur. Heart J., 2009;30(22):2749-57.
Species: Human
Sample Type: Plasma
Application: ELISA Development
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。