抗IL-6 Receptor抗体(Anti-IL-6 Receptor, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27935
IL-6 Receptorに対する抗体(Anti-IL-6 Receptor, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-IL-6 Receptor, Goat-Poly, Biotin
文献数： 3
- 商品コード：BAF1830
- メーカー：RSD
- 包装：50μg
- 価格：¥111,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse IL-6 Rα サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF1830，スタンダードとして#1830-SR-025を用いる。 別名：IL-6 R Genbank No： 3570 Protein Accession No： P22272 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,FCM,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha Leu20-Glu357Accession # P22272 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL-6 R alpha in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human IL‑6 R, recombinant mouse (rm) IL‑6, and rmgp130 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR) |
| Flow Cytometry | 2.5 µg/106 cells | Mouse CD3+ splenocytes |
| Mouse IL-6 R alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse IL‑6 R alpha Antibody (Catalog #AF1830 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑6 R alpha Biotinylated Antibody (Catalog #BAF1830 ) |
| ELISA Standard | Recombinant Mouse IL-6 R alpha (aa 20-357) Protein (Catalog #1830-SR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-6 R alpha | Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51%, and 50% aa identity with rat, human, and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7). |
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References
| Yamasaki, K. et al. (1988) Science 241:825. | |
| Sugita, T. et al. (1990) J. Exp. Med. 171:2001. | |
| Hibi, M. et al. (1990) Cell 63:1149. | |
| Saito, M. et al. (1992) J. Immunol. 148:4066. | |
| Novick, D. et al. (1989) J. Exp. Med. 170:1409. | |
| Jones, S.A. et al. (2001) FASEB J. 15:43. | |
| Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251. | |
| Long Name: | Interleukin 6 Receptor alpha |
| Entrez Gene IDs: | 3570 (Human); 16194 (Mouse) |
| Alternate Names: | CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor |
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Related Research Areas
| B Cell Activation | ||
| Cancer Stem Cell Markers | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| Diabetes | ||
| IL-6 Family | ||
| Immune Regulatory Molecules on Endothelial Cells | ||
| Monocyte Markers | ||
| Multiple Sclerosis and Myelin Related Molecules | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Neuroinflammation | ||
| Obesity | ||
| Receptors in the Jak/STAT Pathway | ||
| T Cell Cytokine Signaling | ||
| Th17 Cells | ||
| Th22 Cells | ||
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Related Product & Information
| Background | IL-6 R alpha |
|---|---|
| background_content | Background: IL-6 R alpha Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51%, and 50% aa identity with rat, human, and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6 R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7). |
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Citations
- Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils.
Authors: Chalaris A, Rabe B, Paliga K, Lange H, Laskay T, Fielding CA, Jones SA, Rose-John S, Scheller J
Blood, 2007;110(6):1748-55.
Species: Mouse
Sample Type: Lavage Fluid
Application: ELISA Development - Transgenic blockade of interleukin 6 transsignaling abrogates inflammation.
Authors: Rabe B, Chalaris A, May U, Waetzig GH, Seegert D, Williams AS, Jones SA, Rose-John S, Scheller J
Blood, 2007;111(3):1021-8.
Species: Mouse
Sample Type: Air Pouch Exudate - Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Type: Plasma
Application: ELISA Development
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