抗IL-4抗体(Anti-IL-4, Canine, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Canine
Label	Biotin
Immunogen	E. coli-derived recombinant canine IL-4 (R&D Systems, Catalog # 754-CL)His25-His132Accession # AAD11563
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects canine IL-4 in Western blots. In this format, approximately 25% cross-reactivity with rhIL-4 is observed, 10% cross-reactivity with rpIL-4 is observed and less than 1% cross-reactivity with rmIL-4 and rrIL-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Canine IL‑4 (Catalog # 754-CL)

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Related Product & Information

Background

IL-4

background_content

Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non‑hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8 - 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

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Citations

1. Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge
   PLoS ONE, 2016;11(8):e0161169.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


2. Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
   PLoS Negl Trop Dis, 2016;10(5):e0004614.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


3. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis.
   Authors: Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M
   BMC Vet Res, 2015;11(0):92.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA detection


4. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
   Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
   Vaccine, 2007;25(21):4223-34.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


5. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
   Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
   Vaccine, 2005;24(4):501-9.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
   Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G
   Vaccine, 2005;23(22):2825-40.
   Species: Canine
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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