抗E-Cadherin抗体(Anti-E-Cadherin, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27894
E-Cadherinに対する抗体(Anti-E-Cadherin, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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[在庫・価格 ：2024年05月06日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-E-Cadherin, Mouse, Goat-Poly, Biotin
文献数： 3
- 商品コード：BAF748
- メーカー：RSD
- 包装：50μg
- 価格：¥113,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse E-Cadherin サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB7482，スタンダードとして#748-EC-050を用いる。 別名：Arc-1 Genbank No： 999 Protein Accession No： P09803 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,IC,IHC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse E-CadherinAsp157-Val709Accession # P09803 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse E-Cadherin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) E-Cadherin, rhN-Cadherin, recombinant mouse (rm) P-Cadherin and rmVE-Cadherin is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse E-Cadherin Fc Chimera (Catalog # 748-EC) |
| Immunohistochemistry | 5-15 µg/mL | Perfusion fixed frozen sections of mouse spinal cord |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Mouse E-Cadherin Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse E‑Cadherin Antibody (Catalog #MAB7482 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse E‑Cadherin Biotinylated Antibody (Catalog #BAF748 ) |
| ELISA Standard | Recombinant Mouse E-Cadherin Fc Chimera Protein, CF (Catalog #748-EC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry E-Cadherin Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/ECadherin_BAF748_Immunocytochemistry_15881.jpg) click image to view larger | E‑Cadherin in A431 Human Cell Line. E‑Cadherin was detected in immersion fixed A431 human skin epidermal cell line using Goat Anti-Mouse E‑Cadherin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: E-Cadherin | Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each. |
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References
| Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123. | |
| Overduin, M. et al. (1995) Science 267:386. | |
| Takeichi, M. (1991) 251:1451. | |
| Entrez Gene IDs: | 999 (Human); 12550 (Mouse); 83502 (Rat) |
| Alternate Names: | Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin |
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Related Research Areas
| Adhesion Molecules | ||
| Adhesion Molecules in Angiogenesis | ||
| Adhesion Molecules on Endothelial Cells | ||
| Adhesive Interactions in Allergy and Asthma | ||
| Cadherin Superfamily | ||
| Cancer Biomarkers | ||
| Cell to Cell Adhesion Disassembly During EMT | ||
| Embryonic and Induced Pluripotent Stem Cell Markers | ||
| Epithelial Cell Markers and Intracellular Molecules | ||
| Epithelial Cell Polarity | ||
| gamma delta T Cells | ||
| Hemangioblasts | ||
| Langerhans and Dermal Dendritic Cells | ||
| Mesenchymal to Epithelial Transition | ||
| Microfilament-associated Proteins | ||
| Surface Ectodermal Cells | ||
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| Immunocytochemistry | |
|---|---|
 | E‑Cadherin in A431 Human Cell Line. E‑Cadherin was detected in immersion fixed A431 human skin epidermal cell line using Goat Anti-Mouse E‑Cadherin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF748) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Related Product & Information
| Background | E-Cadherin |
|---|---|
| background_content | Background: E-Cadherin Epithelial (E) - Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Combined mutation of Apc, Kras and Tgfbr2 effectively drives metastasis of intestinal cancer
Authors: E Sakai, M Nakayama, H Oshima, Y Kouyama, A Niida, S Fujii, A Ochiai, KI Nakayama, K Mimori, Y Suzuki, CP Hong, CY Ock, SJ Kim, M Oshima
Cancer Res., 2017;0(0):.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC-P - Quantifying cell-generated mechanical forces within living embryonic tissues.
Authors: Campas O, Mammoto T, Hasso S, Sperling R, O'Connell D, Bischof A, Maas R, Weitz D, Mahadevan L, Ingber D
Nat Methods, 2014;11(2):183-9.
Species: Mouse
Sample Type: Whole Cells
Application: ICC Fresh - Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
Cell Stem Cell, 2008;3(1):55-68.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
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