抗TNF-α抗体(Anti-TNF-α, Porcine, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Porcine
Label	Biotin
Immunogen	E. coli-derived recombinant porcine TNF-alpha (R&D Systems, Catalog # 690-PT)Arg78-Leu232Accession # P23563
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects porcine TNF-alpha in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Porcine TNF‑ alpha (Catalog # 690-PT)

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Related Product & Information

Background

TNF-alpha

background_content

Background: TNF-alpha Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Porcine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 176 aa extracellular domain (ECD) (3). Within the ECD, porcine TNF-alpha shares 69% - 86% aa sequence identity with bovine, canine, cotton rat, equine, feline, human, mouse, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

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Citations

1. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
   Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
   Intensive Care Med, 2007;33(6):1085-93.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


2. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
   Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
   Cytometry A, 2006;69(5):391-5.
   Species: Porcine
   Sample Type: Serum
   Application: Luminex Development


3. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
   Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
   J. Virol., 2006;80(1):372-82.
   Species: Porcine
   Sample Type: Serum
   Application: ELISA Development

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