抗IL-21抗体(Anti-IL-21, Mouse, Goat-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:27794
IL-21に対する抗体(Anti-IL-21, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2026年04月06日 15時35分現在]
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[在庫・価格 :2026年04月06日 15時35分現在]
Anti-IL-21, Mouse, Goat-Poly, Biotin
文献数: 9
- 商品コード:BAF594
- メーカー:RSD
- 包装:50μg
- 価格:¥91,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
| 説明文 | マッチドペア:Mouse IL-21 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF594,スタンダードとして#594-ML-010を用いる。 別名:IL21 Genbank No: 59067 Protein Accession No: Q9ES17 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)Pro25-Ser146Accession # Q9ES17 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse IL-21 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL‑15, and recombinant human IL‑21 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse IL‑21 (Catalog # 594-ML) |
| Mouse IL-21 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse IL‑21 Antibody (Catalog #AF594 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑21 Biotinylated Antibody (Catalog #BAF594 ) |
| ELISA Standard | Recombinant Mouse IL-21 Protein (Catalog #594-ML ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-21 | Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation. |
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References
| Parrish-Novak, et al. (2000) Nature 408:57. | |
| Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. | |
| Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. | |
| Asao, H. et al. (2001) J. Immunol. 167:1. | |
| Long Name: | Interleukin 21 |
| Entrez Gene IDs: | 59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit) |
| Alternate Names: | IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform |
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Related Research Areas
| B Cell Markers | ||
| Common gamma Chain Receptor Family | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| Isolation, Culture, and Identification of Natural Killer Cells | ||
| Multiple Sclerosis and Myelin Related Molecules | ||
| Natural Killer T (NKT) Cells | ||
| Th17 Cells | ||
| Th2 Cells | ||
| Th22 Cells | ||
| Th9 Cells | ||
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Related Product & Information
| Background | IL-21 |
|---|---|
| background_content | Background: IL-21 Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation. |
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Citations
- Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf
Authors: KM Hooper, W Kong, D Ganea
PLoS ONE, 2017;12(6):e0179184.
Species: Mouse
Sample Type: Cell Culture Supernates - Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
Authors: McGuire HM, Walters S, Vogelzang A
Diabetes, 2011;60(3):867-75.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
PLoS ONE, 2010;5(1):e8720.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development - Regulation of the IL-21 gene by the NF-kappaB transcription factor c-Rel.
Authors: Chen G, Hardy K, Bunting K
J. Immunol., 2010;185(4):2350-9.
Species: Mouse
Sample Type: Serum
Application: ELISA Development - Cytokine-secreting follicular T cells shape the antibody repertoire.
Authors: Reinhardt RL, Liang HE, Locksley RM
Nat. Immunol., 2009;10(4):385-93.
Species: Mouse
Sample Type: Whole Cells
Application: ELISpot Development - Tumor-specific Th17-polarized cells eradicate large established melanoma.
Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood, 2008;112(2):362-73.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
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