抗IL-1β抗体(Anti-IL-1β, Rat, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27724
IL-1βに対する抗体(Anti-IL-1β, Rat, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IL-1β, Rat, Goat-Poly, Biotin |
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[在庫・価格 ：2024年05月19日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-1β, Rat, Goat-Poly, Biotin
文献数： 6
- 商品コード：BAF501
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Rat IL-1β/IL-1F2 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF-501-NA，スタンダードとして#501-RL-010を用いる。 別名：catabolin Genbank No： 3553 Protein Accession No： Q63264 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Rat | 免疫動物 | Goat |
| 交差性 | Rat | 適用 | ELISA,IC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant rat IL-1 beta /IL-1F2 (R&D Systems, Catalog # 501-RL)Val117-Ser268Accession # Q63264 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects rat IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.8% cross-reactivity with recombinant mouse (rm) IL‑1 beta is observed and less than 0.2% cross-reactivity with recombinant human (rh) IL-1 alpha, recombinant rat IL-1 alpha, recombinant porcine (rp) IL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rhIL-1 RA, rmIL-1 RA, rpIL-1 RA, rhIL-1 RII, rmIL-1 RI Fc Chimera, rhIL-1 Rrp2 Fc Chimera, and rhIL-1 RAcP Fc Chimera is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Rat IL-1 beta /IL-1F2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Rat IL‑1 beta /IL‑1F2 Antibody (Catalog #AF-501-NA ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF501 ) |
| ELISA Standard | Recombinant Rat IL-1 beta/IL-1F2 Protein (Catalog #501-RL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry IL-1 beta/IL-1F2 Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_BAF501_Immunocytochemistry__Immunofluorescence_19358.jpg) click image to view larger | IL‑1 beta /IL‑1F2 in Rat Splenocytes.IL‑1 beta /IL‑1F2 was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Rat IL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF501) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-1 beta/IL-1F2 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65-77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta. |
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References
| Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. | |
| Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. | |
| Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. | |
| Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. | |
| Accession # M98820. | |
| Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10. | |
| Long Name: | Interleukin 1 beta |
| Entrez Gene IDs: | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| Alternate Names: | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
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Related Research Areas
| Alpha Granule Soluble Molecules | ||||||||||
| Cancer Biomarkers | ||||||||||
| Conventional/Classical Dendritic Cells | ||||||||||
| Cytokines and Receptors in Allergy and Asthma | ||||||||||
| Cytokines and Receptors in Angiogenesis | ||||||||||
| IL-1 Family | ||||||||||
| Infectious Diseases | ||||||||||
| Inflammatory Mediators | ||||||||||
| Isolation, Culture, and Identification of Natural Killer Cells | ||||||||||
| LTi Cells | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Multiple Sclerosis and Myelin Related Molecules | ||||||||||
| Myeloid-derived Suppressor Cells (MDSC) | ||||||||||
| NCR-ILC3 | ||||||||||
| Neuroinflammation | ||||||||||
| NOD-like Receptors and the Inflammasome | ||||||||||
| Regulatory T Cells (Tregs) | ||||||||||
| T Cell Cytokine Signaling | ||||||||||
| Th17 Cells | ||||||||||
| Th9 Cells | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Rat | Anonymous | 11/07/2017 | Image Details |
 ELISA: Rat IL-1 beta /IL-1F2 Biotinylated Antibody [BAF501]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum |
| Species | Rat |
| Other Experimental Details | This biotinylated antibody paired well with the unbiotinylated version, AF501, in a sandwich ELISA to measure rat IL-1b in serum samples. |
| Immunocytochemistry | |
|---|---|
 | IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Rat IL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF501) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | IL-1 beta/IL-1F2 |
|---|---|
| background_content | Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65-77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues
Molecules, 2016;21(8):.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development - Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infect. Immun., 2008;76(8):3577-86.
Species: Rat
Sample Type: BALF
Application: ELISA Development - In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006;181(1):57-67.
Species: Rat
Sample Type: Plasma
Application: ELISA Development
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