抗VEGF抗体(Anti-VEGF, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27717
VEGFに対する抗体(Anti-VEGF, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-VEGF, Mouse, Goat-Poly, Biotin
文献数： 7
- 商品コード：BAF493
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse VEGF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF-493-NA，スタンダードとして#493-MV-005を用いる。 別名：MVCD1 Genbank No： 7422 Protein Accession No： AAA40547 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Biotin |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164 (R&D Systems, Catalog # 493-MV)Ala27-Arg190Accession # AAA40547 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse VEGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant human (rh) VEGF121, rhVEGF165, and rhVEGF/PlGF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse VEGF164 (Catalog # 493-MV) Recombinant Mouse VEGF120 (Catalog # 494-VE) |
| Mouse VEGF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse VEGF164 Antibody (Catalog #AF-493-NA ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse VEGF Biotinylated Antibody (Catalog #BAF493 ) |
| ELISA Standard | Recombinant Mouse VEGF 164 Protein (Catalog #493-MV ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VEGF | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1 - 3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9). |
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References
| Breier, G. et al. (1992) Development 114:521. | |
| Shima, D.T. et al. (1996) J. Biol. Chem. 271:3877. | |
| Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. | |
| Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. | |
| Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. | |
| Weis, S.M. and D.A. Cheresh (2005) Nature 437:497. | |
| Thurston, G. (2002) J. Anat. 200:575. | |
| Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246. | |
| Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825. | |
| Long Name: | Vascular Endothelial Growth Factor |
| Entrez Gene IDs: | 7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish) |
| Alternate Names: | MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor |
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Related Research Areas
| Adipocytokines | ||
| Adult Neurogenesis | ||
| Airway Inflammation and Remodeling | ||
| Alpha Granule Soluble Molecules | ||
| ALS Related Molecules | ||
| Cancer Biomarkers | ||
| Developmental Factors Implicated in Neurodegeneration | ||
| HIF Transcription Factors | ||
| Inflammatory Mediators | ||
| Macrophage Activation Markers | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Preeclampsia | ||
| VEGF Family | ||
| VEGF Family Ligands | ||
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Related Product & Information
| Background | VEGF |
|---|---|
| background_content | Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1 - 3). It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse (2, 4). Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9). |
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Citations
- Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
Exp Cell Res, 2014;323(1):7-27.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Capture - Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Type: Plasma
Application: Luminex Assay Development - The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
Genes Dev., 2006;20(18):2527-38.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development - Application of plasmid DNA encoding IL-18 diminishes development of herpetic stromal keratitis by antiangiogenic effects.
Authors: Kim B, Lee S, Suvas S, Rouse BT
J. Immunol., 2005;175(1):509-16.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
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