抗Flt-1抗体(Anti-Flt-1, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27697
Flt-1に対する抗体(Anti-Flt-1, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 11時35分現在]
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Anti-Flt-1, Mouse, Goat-Poly, Biotin <Anti-VEGF Receptor 1> |
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[在庫・価格 ：2024年05月18日 11時35分現在]
Anti-Flt-1, Mouse, Goat-Poly, Biotin <Anti-VEGF Receptor 1>
文献数： 4
- 商品コード：BAF471
- メーカー：RSD
- 包装：50μg
- 価格：¥111,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse VEGF R1/Flt-1 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF471，スタンダードとして#471-F1-100を用いる。 別名：EC 2.7.10 Genbank No： 2321 Protein Accession No： P35969 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,FCM,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt-1Ser27-Glu759Accession # P35969 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse VEGF R1 in ELISAs and Western blots. In sandwich ELISAs, less than 7% cross-reactivity with recombinant human VEGF R1 is observed, 4% cross-reactivity with recombinant mouse (rm) VEGF164 is observed, 1% cross-reactivity with recombinant rat VEGF164 is observed, and 0.2% cross-reactivity with rmVEGF R2 and rmVEGF R3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse VEGF R1/Flt‑1 Fc Chimera (Catalog # 471-F1) |
| Flow Cytometry | 2.5 µg/106 cells | bEnd.3 mouse endothelioma cell line |
| Mouse VEGF R1/Flt-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse VEGF R1/Flt‑1 Antibody (Catalog #AF471 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse VEGF R1/Flt‑1 Biotinylated Antibody (Catalog #BAF471 ) |
| ELISA Standard | Recombinant Mouse VEGF R1/Flt-1 Fc Chimera Protein, CF (Catalog #471-F1 ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VEGF R1/Flt-1 | VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tek/Tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, and VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain, and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist. |
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References
| He, Y. et al. (1999) Molecular Endocrinology 13:537. | |
| Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4. | |
| Long Name: | Vascular Endothelial Growth Factor Receptor 1 |
| Entrez Gene IDs: | 2321 (Human); 14254 (Mouse) |
| Alternate Names: | EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1 |
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Related Research Areas
| Airway Inflammation and Remodeling | ||
| Cancer Biomarkers | ||
| Cytokine and Growth Factor Receptors on VSMC | ||
| Endothelial Cell Differentiation Markers | ||
| Endothelial Cell Markers | ||
| Endothelial Progenitor and Endothelial Cell Markers | ||
| HIF Transcription Factors | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Preeclampsia | ||
| Receptor Tyrosine Kinases (RTKs) | ||
| Receptor Tyrosine Kinases (RTKs) in the Akt Pathway | ||
| Receptors in the Jak/STAT Pathway | ||
| VEGF Family | ||
| VEGF Receptors and Regulators on Endothelial Cells | ||
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Related Product & Information
| Background | VEGF R1/Flt-1 |
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| background_content | Background: VEGF R1/Flt-1 VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tek/Tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, and VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain, and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist. |
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Citations
- Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells.
Authors: Shin JY, Yoon IH, Kim JS, Kim B, Park CG
Cell. Immunol., 2009;256(1):72-8.
Species: Mouse
Sample Type: Whole Cells
Application: Flow - Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
Am. J. Pathol., 2006;169(4):1167-82.
Species: Mouse
Sample Type: Tissue Homogenates
Application: ELISA Development
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