抗gp130抗体(Anti-gp130, Mouse, Goat-Poly, Biotin antibody)
掲載日情報:2021/01/28 現在Webページ番号:27694
gp130に対する抗体(Anti-gp130, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 :2024年06月01日 20時55分現在]
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Anti-gp130, Mouse, Goat-Poly, Biotin |
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[在庫・価格 :2024年06月01日 20時55分現在]
Anti-gp130, Mouse, Goat-Poly, Biotin
文献数: 0
- 商品コード:BAF468
- メーカー:RSD
- 包装:50μg
- 価格:¥118,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
マッチドペア:Mouse gp130 サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#MAB4682-500,スタンダードとして#468-MG-100を用いる。 別名:CD130 Genbank No: 3572 Protein Accession No: Q6PDI9 |
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保存条件 | -20℃ | 法規備考 | |
抗原種 | Mouse | 免疫動物 | Goat |
交差性 | Mouse | 適用 | ELISA,FCM,Western Blot |
標識 | Biotin | 性状 | Antigen Affinity Purified |
吸収処理 | クラス | IgG | |
クロナリティ | Polyclonal | フォーマット | |
掲載カタログ |
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製品記事 |
Helper T Cells Marker Antibodies |
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関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Biotin |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse gp130Gln23-Glu617Accession # Q6PDI9 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects mouse gp130 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human gp130, recombinant mouse (rm) IL‑6, and rmIL-6 R is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.1 µg/mL | Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) |
Flow Cytometry | 2.5 µg/106 cells | M1 mouse myeloid leukemia cell line |
Mouse gp130 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse gp130 Antibody (Catalog #MAB4682 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse gp130 Biotinylated Antibody (Catalog #BAF468 ) |
ELISA Standard | Recombinant Mouse gp130 Fc Chimera Protein, CF (Catalog #468-MG ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: gp130 | Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. |
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References
Narazaki, M. et al. (1993) Blood 82:1120. | |
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797. | |
Long Name: | Glycoprotein 130 |
Entrez Gene IDs: | 3572 (Human); 16195 (Mouse); 25205 (Rat) |
Alternate Names: | CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha |
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Related Research Areas
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Related Product & Information
Background | gp130 |
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background_content | Background: gp130 Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R. Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. |
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