抗CXCL10抗体(Anti-CXCL10, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CXCL10/IP‑10/CRG‑2 (R&D Systems, Catalog # 466-CR)Ile22-Pro98Accession # Q548V9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL10/IP‑10/CRG‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IP‑10, recombinant mouse (rm) PF4, rmMIG, rmLIX, and rmI-TAC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)
Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CXCL10/IP‑10/CRG‑2 Antibody (Catalog #MAB466 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog #BAF466 )
ELISA Standard		Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog #466-CR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Related Product & Information

Background

CXCL10/IP-10/CRG-2

background_content

Background: CXCL10/IP-10/CRG-2 The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Activation of stimulator of interferon genes (STING) induces ADAM17-mediated shedding of the immune semaphorin SEMA4D
   Authors: K Motani, H Kosako
   J. Biol. Chem., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


3. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
   Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
   Nat Immunol, 2015;16(8):850-8.
   


4. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
   Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
   J Immunol, 2015;196(3):1227-38.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA detection


5. Colonic patch and colonic SILT development are independent and differentially regulated events.
   Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
   Mucosal Immunol, 2013;6(3):511-21.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


6. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
   Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
   Mol Ther, 2012;20(12):2315-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
   Authors: Meng Y, Efimova EV, Hamzeh KW
   Mol. Ther., 2012;20(5):1046-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
   Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
   Arthritis Rheum., 2006;54(4):1174-83.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
   Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
   Blood, 2005;107(5):2112-22.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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