抗CXCL10抗体(Anti-CXCL10, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CXCL10/IP‑10/CRG‑2 (R&D Systems, Catalog # 466-CR)Ile22-Pro98Accession # Q548V9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL10/IP‑10/CRG‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human IP‑10, recombinant mouse (rm) PF4, rmMIG, rmLIX, and rmI-TAC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)
Immunohistochemistry	5-15 µg/mL	See below
Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CXCL10/IP‑10/CRG‑2 Antibody (Catalog #MAB466 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog #BAF466 )
ELISA Standard		Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog #466-CR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry	click image to view larger	CXCL10/IP‑10/CRG‑2 in Mouse Thymus.CXCL10/IP‑10/CRG‑2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF466) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2	The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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References

Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
Vanguri, P. (1996) J. Neuroimmunol. 56:35.
Sgadari, C. et al. (1996) Blood, 87:3877.
Entrez Gene IDs:	3627 (Human); 15945 (Mouse)
Alternate Names:	C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Related Research Areas

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Infectious Diseases

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

Regulatory T Cells (Tregs)

Activation of stimulator of interferon genes (STING) induces ADAM17-mediated shedding of the immune semaphorin SEMA4D Authors: K Motani, H Kosako J. Biol. Chem., 2018;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB

Immunohistochemistry

CXCL10/IP‑10/CRG‑2 in Mouse Thymus. CXCL10/IP‑10/CRG‑2 was detected in immersion fixed frozen sections of mouse thymus using Goat Anti-MouseCXCL10/IP‑10/CRG‑2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF466) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

CXCL10/IP-10/CRG-2

background_content

Background: CXCL10/IP-10/CRG-2 The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

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Citations

1. Activation of stimulator of interferon genes (STING) induces ADAM17-mediated shedding of the immune semaphorin SEMA4D
   Authors: K Motani, H Kosako
   J. Biol. Chem., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


3. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
   Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
   Nat Immunol, 2015;16(8):850-8.
   


4. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
   Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
   J Immunol, 2015;196(3):1227-38.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA detection


5. Colonic patch and colonic SILT development are independent and differentially regulated events.
   Authors: Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R
   Mucosal Immunol, 2013;6(3):511-21.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


6. Nonviral delivery of small interfering RNA into pancreas-associated immune cells prevents autoimmune diabetes.
   Authors: Leconet W, Petit P, Peraldi-Roux S, Bresson D
   Mol Ther, 2012;20(12):2315-25.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine.
   Authors: Meng Y, Efimova EV, Hamzeh KW
   Mol. Ther., 2012;20(5):1046-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Antagonist of interferon-inducible protein 10/CXCL10 ameliorates the progression of autoimmune sialadenitis in MRL/lpr mice.
   Authors: Hasegawa H, Inoue A, Kohno M, Muraoka M, Miyazaki T, Terada M, Nakayama T, Yoshie O, Nose M, Yasukawa M
   Arthritis Rheum., 2006;54(4):1174-83.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
   Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
   Blood, 2005;107(5):2112-22.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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