抗MIP-2抗体(Anti-MIP-2, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CXCL2/MIP-2 (R&D Systems, Catalog # 452-M2)Ala28-Asn100Accession # P10889
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CXCL2/MIP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) GRO beta, rhGRO gamma, recombinant mouse (rm) C10, rmMIP-1 alpha, rmMIP-1 beta, rhRANTES, and rmKC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 452-M2)
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Mouse CXCL2/MIP-2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog #MAB452 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog #BAF452 )
ELISA Standard		Recombinant Mouse CXCL2/MIP-2 Protein (Catalog #452-M2 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunocytochemistry	click image to view larger	CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Splenocytes.CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry	click image to view larger	CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Spleen.CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3	Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
Entrez Gene IDs:	2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names:	chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROb; gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Related Research Areas

Chemokines and Receptors in Angiogenesis

CXC Chemokines and Receptors

Inflammatory Mediators

Macrophage Activation Markers

Molecules Secreted by VSMC

Neuroinflammation

IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie Cell. Physiol. Biochem., 2017;42(5):1961-1972. Species: Mouse Sample Type: Serum Application: ELISA detection

Immunocytochemistry
	CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Splenocytes. CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	CXCL2/GRO beta /MIP‑2/CINC‑3 in Mouse Spleen. CXCL2/GRO beta /MIP‑2/CINC‑3 was detected in immersion fixed frozen sections of mouse spleen using Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF452) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

CXCL2/GRO beta/MIP-2/CINC-3

background_content

Background: CXCL2/GRO beta/MIP-2/CINC-3 Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

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Citations

1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
   Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
   Cell. Physiol. Biochem., 2017;42(5):1961-1972.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA detection


2. The calcineurin-NFAT axis contributes to host defense during Pseudomonas aeruginosa lung infection
   Authors: Z Pang, RD Junkins, AJ MacNeil, C McCormick, Z Cheng, WM Chen, TJ Lin
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA detection


3. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
   Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
   J Immunol, 2013;190(10):5178-86.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA detection


4. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
   Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
   Am. J. Pathol., 2011;179(6):2730-9.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


5. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
   Microbes Infect., 2009;11(4):524-30.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
   Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
   J. Biol. Chem., 2009;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
   Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
   Immunology, 2008;125(2):272-9.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


8. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
   Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
   Eur. J. Immunol., 2006;36(8):2025-34.
   Species: Mouse
   Sample Type: Tissue Secretion
   Application: ELISA Development


9. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
   Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
   J. Immunol., 2006;177(12):8650-7.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


10. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development

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