抗Eotaxin抗体(Anti-Eotaxin, Mouse, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	E. coli-derived recombinant mouse CCL11/Eotaxin (R&D Systems, Catalog # 420-ME)His24-Pro97Accession # P48298
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL11/Eotaxin in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human CCL11/Eotaxin and recombinant mouse CCL24/Eotaxin-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Immunohistochemistry	5-15 µg/mL	See below
Mouse CCL11/Eotaxin Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CCL11/Eotaxin Antibody (Catalog #AF-420-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL11/Eotaxin Biotinylated Antibody (Catalog #BAF420 )
ELISA Standard		Recombinant Mouse CCL11/Eotaxin Protein (Catalog #420-ME )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry	click image to view larger	CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin	CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

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References

Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Entrez Gene IDs:	6356 (Human); 20292 (Mouse)
Alternate Names:	C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

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Related Research Areas

Adult Neurogenesis

Airway Inflammation and Remodeling

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Chemokines and Receptors in Angiogenesis

Eosinophils

Macrophage Activation Markers

Molecules Secreted by VSMC

Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development

Immunohistochemistry

CCL11/Eotaxin in Mouse Colon. CCL11/Eotaxin was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse CCL11/Eotaxin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

CCL11/Eotaxin

background_content

Background: CCL11/Eotaxin CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

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Citations

1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


2. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
   Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
   Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


3. IL-13Ralpha2 and IL-10 coordinately suppress airway inflammation, airway-hyperreactivity, and fibrosis in mice.
   Authors: Wilson MS, Elnekave E, Mentink-Kane MM, Hodges MG, Pesce JT, Ramalingam TR, Thompson RW, Kamanaka M, Flavell RA, Keane-Myers A, Cheever AW, Wynn TA
   J. Clin. Invest., 2007;117(10):2941-51.
   Species: Mouse
   Sample Type: Serum
   Application: ELISA Development


4. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
   Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
   J. Immunol., 2006;177(3):1967-74.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


5. Sequential ELISA to profile multiple cytokines from small volumes.
   Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
   J. Immunol. Methods, 2005;302(1):172-81.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


6. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
   Authors: Reading PC, Symons JA, Smith GL
   J. Immunol., 2003;170(3):1435-42.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


7. P-selectin mediates IL-13-induced eosinophil transmigration but not eotaxin generation in vivo: a comparative study with IL-4-elicited responses.
   Authors: Larbi KY, Dangerfield JP, Culley FJ, Marshall D, Haskard DO, Jose PJ, Williams TJ, Nourshargh S
   J. Leukoc. Biol., 2003;73(1):65-73.
   Species: Mouse
   Sample Type: Peritoneal Fluid
   Application: ELISA Development


8. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
   Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
   Am. J. Pathol., 2002;160(4):1353-60.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development

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