抗M-CSF抗体(Anti-M-CSF, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27653
M-CSFに対する抗体(Anti-M-CSF, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月01日 09時55分現在]
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[在庫・価格 ：2024年05月01日 09時55分現在]
Anti-M-CSF, Mouse, Goat-Poly, Biotin
文献数： 2
- 商品コード：BAF416
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse M-CSF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB416-500，スタンダードとして#416-ML-010を用いる。 別名：colony stimulating factor 1 (macrophage) Genbank No： 1435 Protein Accession No： Q3U4F9 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse M-CSF (R&D Systems, Catalog # 416-ML)Lys33-Glu262Accession # Q3U4F9 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse M-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with rhM-CSF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse M-CSF (Catalog # 416-ML) |
| Mouse M-CSF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse M‑CSF Antibody (Catalog #MAB416 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse M‑CSF Biotinylated Antibody (Catalog #BAF416 ) |
| ELISA Standard | Recombinant Mouse M-CSF Protein (Catalog #416-ML ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: M-CSF | M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1 - 3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1 - 5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3 - 9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
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References
| Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628. | |
| Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. | |
| Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125. | |
| Ryan, G.R. et al. (2001) Blood 98:74. | |
| Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178. | |
| Nandi, S. et al. (2006) Blood 107:786. | |
| Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026. | |
| Suzu, S. et al. (1992) J. Biol. Chem. 267:16812. | |
| Manos, M.M. (1988) Mol. Cell. Biol. 8:5035. | |
| Koths, K. (1997) Mol. Reprod. Dev. 46:31. | |
| Jang, M-H. et al. (2006) J. Immunol. 177:4055. | |
| DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389. | |
| Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706. | |
| Long Name: | Macrophage Colony Stimulating Factor |
| Entrez Gene IDs: | 1435 (Human); 12977 (Mouse) |
| Alternate Names: | colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930 |
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Related Research Areas
| Cancer Biomarkers | ||
| Dendritic Cell Development | ||
| Gametes and Fertilization | ||
| Inflammatory Mediators | ||
| Molecules Secreted by VSMC | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| SCF, Flt-3 Ligand and M-CSF | ||
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Related Product & Information
| Background | M-CSF |
|---|---|
| background_content | Background: M-CSF M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1 - 3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1 - 5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3 - 9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
追加しました。
Citations
- CSF1 receptor targeting in prostate cancer reverses macrophage-mediated resistance to androgen blockade therapy.
Authors: Escamilla J, Schokrpur S, Liu C, Priceman S, Moughon D, Jiang Z, Pouliot F, Magyar C, Sung J, Xu J, Deng G, West B, Bollag G, Fradet Y, Lacombe L, Jung M, Huang J, Wu L
Cancer Res, 2015;75(6):950-62.
Species: Mouse
Sample Type: Serum
Application: ELISA detection
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