抗MIP-3α抗体(Anti-MIP-3α, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27606
MIP-3αに対する抗体(Anti-MIP-3α, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-MIP-3α, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-MIP-3α, Human, Goat-Poly, Biotin
文献数： 7
- 商品コード：BAF360
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CCL20/MIP-3α サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB360-500，スタンダードとして#360-MP-025を用いる。 別名：beta chemokine exodus-1 Genbank No： 6364 Protein Accession No： P78556 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,IHC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant human CCL20/MIP-3 alpha Ala27-Met96 (Asn95Asp)Accession # P78556 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP‑3 beta, recombinant mouse (rm) MIP‑3 beta, rhMCP-1, rmMIP‑3 alpha, recombinant rat MIP‑3 alpha, and rmJE/MCP-1 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP) |
| Immunohistochemistry | 5-15 µg/mL | See below |
| Human CCL20/MIP-3 alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CCL20/MIP‑3 alpha Antibody (Catalog #MAB360 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog #BAF360 ) |
| ELISA Standard | Recombinant Human CCL20/MIP-3 alpha Protein (Catalog #360-MP ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunohistochemistry |
![Immunohistochemistry CCL20/MIP-3 alpha Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/CCL20_BAF360_Immunohistochemistry_6932.jpg) click image to view larger | CCL20/MIP‑3 alpha in Human Lymph Node.CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Goat Anti-Human CCL20/MIP‑3 alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF360) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CCL20/MIP-3 alpha | CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors. |
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References
| Baba, M. et al. (1997) J. Biol. Chem. 272:14893. | |
| Hromas, R. et al. (1997) Blood 89:3315. | |
| Greaves, D.R. et al. (1997) J. Exp. Med. 186:837. | |
| Entrez Gene IDs: | 6364 (Human); 20297 (Mouse); 29538 (Rat) |
| Alternate Names: | beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 |
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Related Research Areas
| CC Chemokines and Receptors | ||||||||
| Chemokines and Receptors | ||||||||
| Chemokines and Receptors in Angiogenesis | ||||||||
| Macrophage Activation Markers | ||||||||
| Molecules Secreted by VSMC | ||||||||
| Regulatory T Cells (Tregs) | ||||||||
| Th17 Cells | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Excellent | ELISA | Human | Anonymous | 12/07/2017 | ||||
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Summary
| Application | ELISA |
| Sample Tested | Serum |
| Species | Human |
| Other Experimental Details | Good detection in disease samples |
| Immunohistochemistry | |
|---|---|
 | CCL20/MIP‑3 alpha in Human Lymph Node. CCL20/MIP‑3 alpha was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Goat Anti-Human CCL20/MIP‑3 alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF360) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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Related Product & Information
| Background | CCL20/MIP-3 alpha |
|---|---|
| background_content | Background: CCL20/MIP-3 alpha CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy
Authors: G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang
PLoS ONE, 2017;12(5):e0178352.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Type: Urine
Application: Antibody Array Development - Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
Blood, 2007;109(9):3649-57.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Paired synovium and lymph nodes from rheumatoid arthritis patients differ in dendritic cell and chemokine expression.
Authors: Page G, Miossec P
J. Pathol., 2004;204(1):28-38.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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