抗Flk-1抗体(Anti-Flk-1, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # AAC16450
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF R2/KDR/Flk-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse VEGF R2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) VEGF R3 and rhVEGF R1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 357-KD)
Human VEGF R2/KDR Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human VEGF R2/KDR/Flk‑1 Antibody (Catalog #MAB3573 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human VEGF R2/KDR/Flk‑1 Biotinylated Antibody (Catalog #BAF357 )
ELISA Standard		Recombinant Human VEGF R2/KDR Fc Chimera Protein (Catalog #357-KD )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R2/KDR/Flk-1	VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity.

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References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name:	Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs:	3791 (Human); 16542 (Mouse)
Alternate Names:	CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Related Research Areas

Airway Inflammation and Remodeling

Cancer Biomarkers

Cytokine and Growth Factor Receptors on VSMC

Endothelial Cell Differentiation Markers

Endothelial Cell Markers

Endothelial Progenitor and Endothelial Cell Markers

Endothelial Progenitor Cell Markers

Hemangioblasts

Hematopoietic Stem Cell Markers

HIF Transcription Factors

Myeloid-derived Suppressor Cells (MDSC)

Receptor Tyrosine Kinases (RTKs)

Receptor Tyrosine Kinases (RTKs) in the Akt Pathway

Receptors in the Jak/STAT Pathway

Semaphorins, Plexin Receptors, and Related Molecules

VEGF Family

VEGF Receptors and Regulators on Endothelial Cells

Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants Authors: J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo,, M Ayala Avil BMC Immunol., 2017;18(1):39. Species: Human Sample Type: Serum Application: ELISA detection

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Related Product & Information

Background

VEGF R2/KDR/Flk-1

background_content

Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity.

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Citations

1. Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants
   Authors: J Sánchez Ra, Y Morera Día, M Bequet-Rom, F Hernández-, KH Selman-Hou, A de la Torr, ER Santiesteb, Y Martín Bau, CH Bermúdez B, J de la Torr, JV Gavilondo,, M Ayala Avil
   BMC Immunol., 2017;18(1):39.
   Species: Human
   Sample Type: Serum
   Application: ELISA detection


2. Specific humoral and cellular immune responses in cancer patients undergoing chronic immunization with a VEGF-based therapeutic vaccine
   Authors: Y Morera, J Sánchez, M Bequet-Rom, KH Selman-Hou, A de la Torr, F Hernández-, Y Martín, A Garabito, J Piñero, C Bermúdez, J de la Torr, M Ayala, JV Gavilondo
   Vaccine, 2017;0(0):.
   Application: ELISA detection


3. Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
   Br. J. Cancer, 2012;107(10):1702-13.
   Species: Human
   Sample Type: Cell Lysates
   Application: TaqMan Protein Assay


4. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
   Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
   Br. J. Cancer, 2006;94(4):524-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis.
   Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
   Exp. Cell Res., 2004;299(1):68-78.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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