抗CSF-1 Receptor抗体(Anti-CSF-1 Receptor, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27580
CSF-1 Receptorに対する抗体(Anti-CSF-1 Receptor, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月01日 17時35分現在]
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Anti-CSF-1 Receptor, Human, Goat-Poly, Biotin <Anti-M-CSF Receptor> |
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[在庫・価格 ：2024年05月01日 17時35分現在]
Anti-CSF-1 Receptor, Human, Goat-Poly, Biotin <Anti-M-CSF Receptor>
文献数： 1
- 商品コード：BAF329
- メーカー：RSD
- 包装：50μg
- 価格：¥114,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human M-CSF R/CD115 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB3292-500，スタンダードとして#329-MR-100を用いる。 別名：CD115 Genbank No： 1436 Protein Accession No： CAA27300 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,FCM,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Biotin |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115Ile20-Glu512Accession # CAA27300 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human M-CSF R/CD115 in ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human M-CSF R/CD115 Fc Chimera (Catalog # 329-MR) |
| Flow Cytometry | 0.25 µg/106 cells | Human peripheral blood monocytes |
| Human M-CSF R/CD115 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human M-CSF R/CD115 Antibody (Catalog #MAB3292 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human M-CSF R/CD115 Biotinylated Antibody (Catalog #BAF329 ) |
| ELISA Standard | Recombinant Human M-CSF R/CD115 Fc Chimera Protein (Catalog #329-MR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: M-CSF R/CD115 | M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells, and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation, and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased |
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References
| deParseval, N. et al. (1993) Nucleic Acids Res. 21:750. | |
| Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311. | |
| Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. | |
| Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88. | |
| Rovida, E. et al. (2001) J. Immunol. 166:1583. | |
| Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128. | |
| Dai, X. et al. (2002) Blood 99:111. | |
| Faccio, R. et al. (2003) J. Clin. Invest. 111:749. | |
| Li, M. et al. (2004) J. Neurochem. 91:623. | |
| Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442. | |
| Long Name: | Macrophage Colony Stimulating Factor Receptor |
| Entrez Gene IDs: | 1436 (Human) |
| Alternate Names: | CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms |
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Related Research Areas
| Common Macrophage Markers | ||
| Dendritic Cell Development | ||
| Monocyte Markers | ||
| Mouse Tissue-Resident Macrophage Markers | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Receptor Tyrosine Kinases (RTKs) in the Akt Pathway | ||
| Receptors in the Jak/STAT Pathway | ||
| SCF, Flt-3 Ligand and M-CSF | ||
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Related Product & Information
| Background | M-CSF R/CD115 |
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| background_content | Background: M-CSF R/CD115 M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells, and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation, and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increasedM-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10). |
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Citations
- Impaired long-term expansion and self-renewal potential of pediatric acute myeloid leukemia-initiating cells by PTK787/ZK 222584.
Authors: Weidenaar A, Ter Elst A, Kampen K, Meeuwsen-de Boer T, Kamps W, Schuringa J, de Bont E
Mol Cancer Res, 2013;11(4):339-48.
Species: Human
Sample Type: Whole Cells
Application: Flow
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