抗VEGF抗体(Anti-VEGF, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27560
VEGFに対する抗体(Anti-VEGF, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-VEGF, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-VEGF, Human, Goat-Poly, Biotin
文献数： 25
- 商品コード：BAF293
- メーカー：RSD
- 包装：50μg
- 価格：¥120,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human/Primate VEGF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB293-500または#MAB293R-100，スタンダードとして#293-VE-010を用いる。 別名：MVCD1 Genbank No： 7422 Protein Accession No： AAV38412 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker Myeloid-derived Suppressor Cell Marker |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human, Primate |
|---|---|
| Label | Biotin |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165 (R&D Systems, Catalog # 293-VE)Ala27-Arg191Accession # AAV38412 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human VEGF165 in ELISAs and Western blots. In sandwich immunoassays, less than 15% cross-reactivity with recombinant human (rh) VEGF/PlGF is observed and less than 0.1% cross-reactivity with recombinant mouse (rm) PlGF-2, rhFlt-1, rhVEGF R2, rhPlGF, rhVEGF‑C, rhVEGF-D, and rmVEGF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human VEGF165 (Catalog # 293-VE) |
| Human/Primate VEGF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate VEGF Antibody (Catalog #MAB293 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human/Primate VEGF165 Biotinylated Antibody (Catalog #BAF293 ) |
| ELISA Standard | Recombinant Human VEGF 165 Protein (Catalog #293-VE ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VEGF | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1 - 3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9). |
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References
| Leung, D.W. et al. (1989) Science 246:1306. | |
| Keck, P.J. et al. (1989) Science 246:1309. | |
| Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. | |
| Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. | |
| Pan, Q. et al. (2007) J. Biol. Chem. 282:24049. | |
| Weis, S.M. and D.A. Cheresh (2005) Nature 437:497. | |
| Thurston, G. (2002) J. Anat. 200:575. | |
| Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246. | |
| Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825. | |
| Long Name: | Vascular Endothelial Growth Factor |
| Entrez Gene IDs: | 7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish) |
| Alternate Names: | MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor |
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Related Research Areas
| Adipocytokines | ||||||||||
| Adult Neurogenesis | ||||||||||
| Airway Inflammation and Remodeling | ||||||||||
| Alpha Granule Soluble Molecules | ||||||||||
| ALS Related Molecules | ||||||||||
| Cancer Biomarkers | ||||||||||
| Developmental Factors Implicated in Neurodegeneration | ||||||||||
| HIF Transcription Factors | ||||||||||
| Inflammatory Mediators | ||||||||||
| Macrophage Activation Markers | ||||||||||
| Myeloid-derived Suppressor Cells (MDSC) | ||||||||||
| Preeclampsia | ||||||||||
| VEGF Family | ||||||||||
| VEGF Family Ligands | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | ELISA | Human | Anonymous | 11/16/2017 | Image Details |
 ELISA: Human/Primate VEGF165 Biotinylated Antibody [BAF293]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | This antibody was used as the detection in an ELISA targeting VEGF in human serum samples. |
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Related Product & Information
| Background | VEGF |
|---|---|
| background_content | Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1 - 3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
PLoS ONE, 2017;12(4):e0175712.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells
Authors: MG Atzori, L Tentori, F Ruffini, C Ceci, L Lisi, E Bonanno, M Scimeca, E Eskilsson, T Daubon, H Miletic, L Ricci Viti, R Pallini, P Navarra, R Bjerkvig, S D'Atri, PM Lacal, G Graziani
J. Exp. Clin. Cancer Res., 2017;36(1):106.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
PLoS ONE, 2017;12(9):e0184039.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion
PLoS ONE, 2016;11(7):e0159532.
Species: Human
Sample Type: Cell Lysates
Application: WB - Ellagic Acid Inhibits Bladder Cancer Invasiveness and In Vivo Tumor Growth
Authors: Claudia Ceci
Nutrients, 2016;8(11):.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Dectection - Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
Lab Invest, 2014;94(7):716-25.
Species: Human
Sample Type: Cell Lysates
Application: WB - Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
Authors: Ruffini F, Tentori L, Dorio A, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal P
Oncol Rep, 2013;30(6):2887-96. - Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
Br. J. Cancer, 2012;107(10):1702-13.
Species: Human
Sample Type: Cell Lysates
Application: TaqMan Protein Assay - Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation.
Authors: Soman C, Giorgio T,
Nanomedicine, 2009;5(4):402-9.
Species: Human
Sample Type: Recombinant Protein
Application: Quantum Dot - Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008;7(6):2406-14.
Species: Human
Sample Type: Serum
Application: ELISA Microarray Development
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