抗IL-15抗体(Anti-IL-15, Human, Mouse-Mono, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Primate
Label	Biotin
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34593
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-15 in ELISAs and Western blots. In ELISAs, this antibody shows no cross-reactivity or intereference with recombinant human (rh) IL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-2, rhIL-2 R alpha, rhIL-2 R beta, rhIL-2 R gamma, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-4 R, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-7 R, rhIL-8, rhIL-9, rmIL-9, rmIL-13, and rhTGF-beta RII.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-IL)
Human/Primate IL-15 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Primate IL‑15 Antibody (Catalog #MAB647 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human/Primate IL‑15 Biotinylated Antibody (Catalog #BAM247 )
ELISA Standard		Recombinant Human IL-15 Protein (Catalog #247-IL )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

目次に戻る

References

Grabstein, K. et al. (1994) Science 264:965.
Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. 94:14444.
Giri, J.G. et al. (1995) EMBO 14:3654.
Giri, J. et al. (1994) EMBO J. 13:2822.
Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
Dubois, S. et al. (2002) Immunity 17:537.
Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
Mortier, E. et al. (2004) J. Immunol. 173:1681.
Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name:	Interleukin 15
Entrez Gene IDs:	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Alternate Names:	IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

目次に戻る

Related Research Areas

Show

Common gamma Chain Receptor Family
Group 1 ILCs
Isolation, Culture, and Identification of Natural Killer Cells
Macrophage Activation Markers
Molecules Secreted by VSMC
Natural Killer T (NKT) Cells
Highly potent anti-CD20-RLI immunocytokine targeting established human B lymphoma in SCID mouse. Authors: Vincent M, Teppaz G, Lajoie L, Sole V, Bessard A, Maillasson M, Loisel S, Bechard D, Clemenceau B, Thibault G, Garrigue-Antar L, Jacques Y, Quemener A MAbs, 2014;6(4):1026-37. Species: Human Sample Type: Plasma Application: ELISA Development
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Human	Anonymous	11/20/2017			Image Details	ELISA: Human/Primate IL-15 Biotinylated Antibody [BAM247].

目次に戻る

Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	This antibody served as the detection in an ELISA with MAB647 as the capture.

目次に戻る

Related Product & Information

Background

IL-15

background_content

Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

目次に戻る

Citations

1. Highly potent anti-CD20-RLI immunocytokine targeting established human B lymphoma in SCID mouse.
   Authors: Vincent M, Teppaz G, Lajoie L, Sole V, Bessard A, Maillasson M, Loisel S, Bechard D, Clemenceau B, Thibault G, Garrigue-Antar L, Jacques Y, Quemener A
   MAbs, 2014;6(4):1026-37.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


2. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


3. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
   Authors: Pfleger C, Schloot N, ter Veld F
   J. Immunol. Methods, 2007;329(1):214-8.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


4. Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
   Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK
   Immunology, 2000;99(3):411-7.
   Species: Human
   Sample Type: Amniotic Fluid
   Application: ELISA Development

目次に戻る