抗TGF-β Receptor Ⅱ抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly, Biotin antibody)

掲載日情報:2021/01/28 現在Webページ番号:27514

TGF-β Receptor Ⅱに対する抗体(Anti-TGF-β Receptor Ⅱ, Human, Goat-Poly, Biotin )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年02月16日 00時00分現在]

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Anti-TGF-β Receptor II, Human, Goat-Poly, Biotin
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
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説明文
マッチドペア:Human TGF-β RII サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF-241-NA,スタンダードとして#241-R2-025を用いる。
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Biotin
交差性 Human 適用 ELISA,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2025年02月16日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-TGF-β Receptor II, Human, Goat-Poly, Biotin

文献数: 2

説明文 マッチドペア:Human TGF-β RII サンドイッチELISAの検出用抗体として利用可能,補足用抗体として#AF-241-NA,スタンダードとして#241-R2-025を用いる。
別名:AAT3
Genbank No: 7048
Protein Accession No: P37173
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ELISA,Western Blot
標識 Biotin 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Helper T Cells Marker Antibodies
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityHuman
LabelBiotin
ImmunogenMouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RIIIle24-Asp159Accession # P37173
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human TGF-beta  RII in ELISAs and Western blots. In sandwich immunoassays, approximately 25% cross-reactivity with recombinant human (rh) TGF-beta RIIb is observed and less than 0.05% cross-reactivity with rhTGF-beta RI, rhTGF-beta RIII, recombinant mouse TGF-beta RII, and rhTGF-beta 1 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Human TGF-beta  RII (Catalog # 241-R2)
Human TGF-beta RII Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)0.2-0.8 µg/mL Human TGF‑ beta  RII Antibody (Catalog #AF-241-NA )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Human TGF‑ beta  RII Biotinylated Antibody (Catalog #BAF241 )
ELISA Standard  Recombinant Human TGF-beta RII Protein (Catalog #241-R2 )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.Preparation and Storage
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

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References

Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name:Transforming Growth Factor beta Receptor II
Entrez Gene IDs:7048 (Human); 21813 (Mouse)
Alternate Names:AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

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Related Research Areas

Airway Inflammation and Remodeling
Cytokine and Growth Factor Receptors on VSMC
Cytokines and Receptors in Angiogenesis
Preeclampsia
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
TGF-beta Family
TGF-beta Superfamily
Th17 Cells
Th22 Cells
Th9 Cells
  1. Ad5/48 hexon oncolytic virus expressing sTGFbetaRIIFc produces reduced hepatic and systemic toxicities and inhibits prostate cancer bone metastases.
    Authors: Xu W, Zhang Z, Yang Y, Hu Z, Wang C, Morgan M, Wu Y, Hutten R, Xiao X, Stock S, Guise T, Prabhakar B, Brendler C, Seth P
    Mol Ther, 2014;22(8):1504-17.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA detection


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Related Product & Information

BackgroundTGF-beta RII
background_contentBackground:
TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors. The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Ad5/48 hexon oncolytic virus expressing sTGFbetaRIIFc produces reduced hepatic and systemic toxicities and inhibits prostate cancer bone metastases.
    Authors: Xu W, Zhang Z, Yang Y, Hu Z, Wang C, Morgan M, Wu Y, Hutten R, Xiao X, Stock S, Guise T, Prabhakar B, Brendler C, Seth P
    Mol Ther, 2014;22(8):1504-17.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA detection

  2. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
    Authors: La Cava A, Ebling FM, Hahn BH
    J. Immunol., 2004;173(5):3542-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow



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