抗IL-6 Receptor抗体(Anti-IL-6 Receptor, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27502
IL-6 Receptorに対する抗体(Anti-IL-6 Receptor, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時35分現在]
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[在庫・価格 ：2025年04月26日 20時35分現在]
Anti-IL-6 Receptor, Human, Goat-Poly, Biotin
文献数： 8
- 商品コード：BAF227
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human IL-6 Rα サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB227-500，スタンダードとして#227-SR-025を用いる。 別名：IL-6 R Genbank No： 3570 Protein Accession No： P08887 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha (R&D Systems, Catalog # 227-SR)Leu20-Asp358Accession # P08887 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human IL-6 R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑6, recombinant mouse IL-6, recombinant porcine IL-6, and rhgp130 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human IL‑6 R alpha (Catalog # 227-SR) |
| Human IL-6 R alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human IL‑6 R alpha Antibody (Catalog #MAB227R ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human IL‑6 R alpha Biotinylated Antibody (Catalog #BAF227 ) |
| ELISA Standard | Recombinant Human IL-6 R alpha Protein (Catalog #227-SR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-6 R alpha | The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7). |
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References
| Yamasaki et al. (1988) Science 241:825. | |
| Baumann et al. (1990) J. Biol. Chem. 265:19853. | |
| Hibi et al. (1990) Cell 63:1149. | |
| Schooltink et al. (1991) Eur. J. Biochem. 277:659. | |
| Novick et al., (1989) J. Exp. Med. 170:1409. | |
| Yasukawa et al. (1990) J. Biochem. 108:673. | |
| Saito et al. (1991) J. Immunology 147:168. | |
| Long Name: | Interleukin 6 Receptor alpha |
| Entrez Gene IDs: | 3570 (Human); 16194 (Mouse) |
| Alternate Names: | CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor |
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Related Research Areas
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Summary
| Application | ELISA |
| Sample Tested | EDTA Plasma |
| Species | Human |
| Other Experimental Details | Human IL-6 receptor alpha was measured in human plasma samples using an immunoassay comprising of this antibody and MAB227. This antibody was used as the detection and the MAB227 was used as the solid phase capture molecule. |
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Related Product & Information
| Background | IL-6 R alpha |
|---|---|
| background_content | Background: IL-6 R alpha The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7). |
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Citations
- Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering.
Authors: Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K
J Immunol, 2015;195(7):3198-205.
Species: Human
Sample Type: Protein
Application: ELISA Development - A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor.
Authors: Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J
J Biol Chem, 2014;289(23):16336-48.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA detection - Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
Authors: Wood MW, Breitschwerdt EB, Gookin JL
Infect. Immun., 2011;79(2):708-15.
Species: Canine
Sample Type: Whole Tissue
Application: IHC Frozen - trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004;80(6):1521-5.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002;169(9):5244-51.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001;14(6):705-14.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
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