抗CXCL16抗体(Anti-CXCL16, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Primate
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CXCL16Asn49-Pro137Accession # NP_071342
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CXCL16 in ELISAs and Western blots. In ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) CXCL16, rm6Ckine, recombinant human (rh) BLC, rhCTACK, rhIL-8, rhPF4, and rhLymphotactin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX)
Flow Cytometry	0.25 µg/106 cells	Human monocyte-derived dendritic cells
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Human/Primate CXCL16 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Human/Primate CXCL16 Antibody (Catalog #AF976 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human/Primate CXCL16 Biotinylated Antibody (Catalog #BAF976 )
ELISA Standard		Recombinant Human CXCL16 (Histidine-tagged) Protein (Catalog #1164-CX )
Neutralization	Measured by its ability to neutralize CXCL16-induced chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nat. Immunol.The Neutralization Dose (ND50) is typically 0.05-0.25 μg/mL in the presence of 20 ng/mL Recombinant Human CXCL16 Chemokine Domain.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody.Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) induces chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976). The ND50 is typically 0.05-0.25 μg/mL.
Immunohistochemistry	click image to view larger	CXCL16 in Human Lymph Node.CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry	click image to view larger	CXCL16 in Human Lymphoma.CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL16	CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

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References

Matloubian, M. et al. (2000) Nature Immun. 1:298.
Shimaoka, T. et al. (2000) J. Biol. Chem. 275:40663.
Wilbanks, A. et al. (2001) J. Immun. 166:5145.
Entrez Gene IDs:	58191 (Human); 66102 (Mouse)
Alternate Names:	chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

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Related Research Areas

Show

Chemokines and Receptors
Chemokines and Receptors in Angiogenesis
Infectious Diseases
Macrophage Activation Markers
Molecules Secreted by VSMC
Natural Killer T (NKT) Cells
Scavenger Receptors
Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease Authors: X Qu, X Dang, W Wang, Y Li, D Xu, D Shang, Y Chang Mediators Inflamm., 2018;2018(0):7501851. Application: Neut
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ICC/IF	Human	Anonymous	06/16/2017			Image Details	Immunocytochemistry/Immunofluorescence: Human/Primate CXCL16 Antibody [AF976].

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Summary

Application	Immunocytochemistry/Immunofluorescence
Sample Tested	OVARIAN CELL LINE,OVCAR5 CELL LINE
Species	Human

Neutralization
	Chemotaxis Induced by CXCL16 and Neutralization by Human CXCL16 Antibody. Recombinant Human CXCL16 Chemokine Domain (Catalog # 976-CX) induces chemotaxis in BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human CXCL16 Chemokine Domain (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976). The ND50 is typically0.05-0.25 μg/mL.
Immunohistochemistry
	CXCL16 in Human Lymph Node. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	CXCL16 in Human Lymphoma. CXCL16 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Primate CXCL16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF976) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	58191 (Human); 66102 (Mouse)
Background	CXCL16
background_content	Background: CXCL16 CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Together with Fractalkine (CX3CL1), CXCL16 constitute the only two transmembrane chemokines within the superfamily. The gene for human CXCL16 predicts a 273 amino acid (aa) residue precursor protein with a putative signal peptide, a CXC chemokine domain, a mucin-like spacer region, a transmembrane domain and a cytoplasmic domain with a potential tyrosine phosphorylation and SH2 protein-binding site. Mouse and human CXCL16 share 70% aa sequence similarity within their chemokine domains and 49% overall aa sequence identity. By northern blot analysis, CXCL16 expression is detected in various human organs except for brain, bone marrow, skeletal muscle or colon. By flow cytometry, CXCL16 has been detected on the surface CD19+ B cells, CD14+ monocytes/macrophages, and CD11c+ splenic and lymph node dendritic cells. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein. The functional receptor for CXCL16 has been identified as CXCR6 (also known as Bonzo, STRL33 or TYMSTR), a receptor previously shown to be a co-receptor for HIV entry. CXCL16 has also been independently cloned and named SRPSOX (scavenger receptor that binds phosphatidylserine and oxidized lipoprotein). It was shown to be a specific receptor for OxLDL but not LDL or acetyl-LDL.

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Citations

1. Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease
   Authors: X Qu, X Dang, W Wang, Y Li, D Xu, D Shang, Y Chang
   Mediators Inflamm., 2018;2018(0):7501851.
   Application: Neut


2. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
   Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
   PLoS ONE, 2014;9(6):e99056.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA.
   Authors: Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P
   PLoS ONE, 2012;7(8):e41952.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Overexpression of CXCL16 and its receptor CXCR6/Bonzo promotes growth of human schwannomas.
   Authors: Held-Feindt J, Rehmke B, Mentlein R, Hattermann K, Knerlich F, Hugo HH, Ludwig A, Mehdorn HM
   Glia, 2008;56(7):764-74.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis.
   Authors: Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C
   Clin. Immunol., 2008;129(2):268-76.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Expression and potential function of the CXC chemokine CXCL16 in pancreatic ductal adenocarcinoma.
   Authors: Wente MN, Gaida MM, Mayer C, Michalski CW, Haag N, Giese T, Felix K, Bergmann F, Giese NA, Friess H
   Int. J. Oncol., 2008;33(2):297-308.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. CXCL16-mediated cell recruitment to rheumatoid arthritis synovial tissue and murine lymph nodes is dependent upon the MAPK pathway.
   Authors: Ruth JH, Haas CS, Park CC, Amin MA, Martinez RJ, Haines GK, Shahrara S, Campbell PL, Koch AE
   Arthritis Rheum., 2006;54(3):765-78.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
   Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
   Arthritis Rheum., 2006;54(8):2402-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. EPHB4 regulates chemokine-evoked trophoblast responses: a mechanism for incorporating the human placenta into the maternal circulation.
   Authors: Red-Horse K, Kapidzic M, Zhou Y, Feng KT, Singh H, Fisher SJ
   Development, 2005;132(18):4097-106.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC

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