抗MMP-9抗体(Anti-MMP-9, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human MMP‑9 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12 and -13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018)
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	MMP‑9 in MMP‑9 transfected NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	MMP‑9 in Human Liver Cancer Tissue. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Matrix Metalloproteinase 9
Background	MMP-9
background_content	Background: MMP-9 The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

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Citations

1. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
   Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
   J Immunol, 2015;195(5):2429-41.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


2. Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
   Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
   J. Immunol., 2006;177(7):4861-9.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
   Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
   Anal. Biochem., 2005;338(2):294-8.
   Species: Human
   Sample Type: Plasma
   Application: WB

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