抗MMP-9抗体(Anti-MMP-9, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9Ala20-Pro730Accession # P41245
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	.25 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse MMP‑9 (Catalog # 909-MM), see our available Western blot detection antibodies

Western Blot
	Detection of Mouse MMP‑9 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP‑9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	MMP‑9 in Rat Brain. MMP‑9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse MMP‑9 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP‑9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Matrix Metalloproteinase 9
Background	MMP-9
background_content	Background: MMP-9 Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin, and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages, and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9(Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

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Citations

1. Immunodominant fragments of myelin basic protein initiate T cell-dependent pain.
   J Neuroinflammation, 2102;9(0):119.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
   Authors: LQ Liu, HS Li, SP Nie, MY Shen, JL Hu, MY Xie
   Int J Mol Sci, 2018;19(2):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice
   Authors: ND Thiele, JW Wirth, D Steins, AC Koop, H Ittrich, AW Lohse, J Kluwe
   Sci Rep, 2017;7(1):714.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. Endotoxemia shifts neutrophils with TIMP-free gelatinase B/ MMP-9 from bone marrow to periphery and induces systematic upregulation of TIMP-1
   Authors: J Vandooren, W Swinnen, E Ugarte-Ber, L Boon, D Dorst, E Martens, G Opdenakker
   Haematologica, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


5. Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization Controls Endochondral Ossification through Hyaluronan Metabolism
   Authors: M Shimoda, H Yoshida, S Mizuno, T Hirozane, K Horiuchi, Y Yoshino, H Hara, Y Kanai, S Inoue, M Ishijima, Y Okada
   Am. J. Pathol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


6. Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B
   Sci Rep, 2016;6(0):29852.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Bexarotene targets autophagy and is protective against thromboembolic stroke in aged mice with tauopathy
   Sci Rep, 2016;6(0):33176.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
   Physiol Rep, 2016;4(23):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice.
   Authors: Kato H, Duarte S, Liu D, Busuttil R, Coito A
   PLoS ONE, 2015;10(9):e0137642.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


10. Cytomegalovirus initiates infection selectively from high-level beta1 integrin-expressing cells in the brain.
    Authors: Kawasaki H, Kosugi I, Sakao-Suzuki M, Meguro S, Arai Y, Tsutsui Y, Iwashita T
    Am J Pathol, 2015;185(5):1304-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB

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