抗IL-18 Receptor抗体(Anti-IL-18 Receptor, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5Glu20-Arg329Accession # Q13478
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR)
Flow Cytometry	2.5 µg/106 cells	Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4.

Immunohistochemistry
	IL‑18 R alpha /IL‑1 R5 in Human Skin. IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody. Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.

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Related Product & Information

Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Background	IL-18 R alpha/IL-1 R5
background_content	Background: IL-18 R alpha/IL-1 R5 Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

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Citations

1. MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
   Authors: A Gibbs, E Leeansyah, A Introini, D Paquin-Pro, K Hasselrot, E Andersson, K Broliden, JK Sandberg, A Tjernlund
   Mucosal Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IF


2. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
   Sci Rep, 2016;6(0):26757.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
   Authors: Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
   Blood, 2011;117(4):1250-9.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
   Authors: Sallmon H, Hoene V, Weber SC, Dame C
   J. Interferon Cytokine Res., 2010;30(2):55-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
   Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
   Ann. Neurol., 2005;57(1):50-9.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB

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