100th つないで、進む FRONTIERS IN LIFE SCIENCE

検索の使い方

抗CD80抗体(Anti-CD80, Mouse, Goat-Poly antibody)

掲載日情報：2021/01/28 現在Webページ番号：27255

アールアンドディー システムズ /
R&D Systems, Inc.
[メーカー略称：RSD]

CD80に対する抗体(Anti-CD80, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。

カートに商品を
追加しました。

価格

[在庫・価格 ：2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
 納期 文献数
Anti-CD80, Mouse, Goat-Poly <Anti-B7-1>
  • 使用文献
  • メーカーサイト
  • お問い合わせ
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 5
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
マッチドペア：Mouse B7-1/CD80 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF740，スタンダードとして#740-B1-100を用いる。
別名：Activation B7-1 antigen
Genbank No： 941
Protein Accession No： Q00609
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 ELISA,FCM,IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Mouse B7-1/CD80 Affinity Purified Polyclonal Ab
  • 使用文献
  • メーカーサイト
  • お問い合わせ
 2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
  • 使用文献
  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状：溶液または凍結乾燥
別名：Activation B7-1 antigen
Genbank No： 941
Protein Accession No： Q00609
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 ELISA,FCM,IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 ：2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CD80, Mouse, Goat-Poly <Anti-B7-1>

文献数： 5

説明文 マッチドペア：Mouse B7-1/CD80 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF740，スタンダードとして#740-B1-100を用いる。
別名：Activation B7-1 antigen
Genbank No： 941
Protein Accession No： Q00609
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 ELISA,FCM,IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
  • 使用文献
  • メーカーサイト
  • お問い合わせ

Anti-Mouse B7-1/CD80 Affinity Purified Polyclonal Ab

文献数： 1

説明文 ※受注発注品。形状：溶液または凍結乾燥
別名：Activation B7-1 antigen
Genbank No： 941
Protein Accession No： Q00609
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Mouse 適用 ELISA,FCM,IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社）
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
  • 使用文献
  • メーカーサイト
  • お問い合わせ



目次に戻る

カートに商品を
追加しました。

Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse B7‑1/CD80Asp37-Lys245Accession # Q00609
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects mouse B7‑1/CD80 in ELISAs and Western blots.


目次に戻る

カートに商品を
追加しました。

Applications and Data

 Recommended
Concentration		Sample
Western Blot1 µg/mLSee below
Flow Cytometry0.25 µg/106 cellsSee below
CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry5-15 µg/mLSee below
Mouse B7-1/CD80 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)0.2-0.8 µg/mL Mouse B7‑1/CD80 Antibody (Catalog #AF740 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Mouse B7‑1/CD80 Biotinylated Antibody (Catalog #BAF740 )
ELISA Standard  Recombinant Mouse B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog #740-B1 )
NeutralizationMeasured by its ability to neutralize B7‑1/CD80-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

目次に戻る

カートに商品を
追加しました。

Data Examples

Western Blot Western Blot B7-1/CD80 Antibody [Unconjugated]
click image to view larger		 Detection of Mouse B7‑1/CD80 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry Flow Cytometry B7-1/CD80 Antibody [Unconjugated]
click image to view larger		 Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry Immunocytochemistry B7-1/CD80 Antibody [Unconjugated]
click image to view larger

B7‑1/CD80 in Mouse Splenocytes.

B7‑1/CD80 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization Neutralization B7-1/CD80 Antibody [Unconjugated]
click image to view larger		IL‑2 secretion Induced by
B7‑1/CD80 and Neutralization by Mouse
B7‑1/CD80 Antibody.Recombinant Mouse
B7‑1/CD80 Fc Chimera (Catalog # 740-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant Mouse
B7‑1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND50 is typically
0.15‑0.6 µg/mL.
ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the
down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse
B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

目次に戻る

カートに商品を
追加しました。

References

Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) Science 262:909.
Freeman, G. et al. (1991) J. Exp. Med. 174:625.
Selvakumar, A. et al. (1993) Immunogenetics 38:292.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs:941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names:Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

目次に戻る

カートに商品を
追加しました。

Related Research Areas

B Cell Activation
B Cell Markers
Common Macrophage Markers
Co-stimulatory and Co-inhibitory Molecules
Co-stimulatory Molecules Associated with Tfh Cells
Dendritic Cell Co-stimulatory Molecules
Dendritic Cell Development
Macrophage Activation Markers
Mouse Tissue-Resident Macrophage Markers
Multiple Myeloma
Myeloid-derived Suppressor Cells (MDSC)
Regulatory T Cells (Tregs)
T Cell Antigen Recognition
  1. High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells
    Authors: VK Clements, T Long, R Long, C Figley, DMC Smith, S Ostrand-Ro
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow


Western Blot
Detection of Mouse B7‑1/CD80 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7‑1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
Detection of B7‑1/CD80 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes either treated with 200 ng/mL LPS (filled histogram) or unstimulated (open histogram) were stained with Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
B7‑1/CD80 in Mouse Splenocytes.
B7‑1/CD80 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
IL‑2 secretion Induced byB7‑1/CD80 and Neutralization by MouseB7‑1/CD80 Antibody.
Recombinant MouseB7‑1/CD80 Fc Chimera (Catalog # 740-B1) co-stimulates IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL‑2 Quantikine ELISA Kit (Catalog # D2050). IL‑2 secretion elicited by Recombinant MouseB7‑1/CD80 Fc Chimera (0.1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF740). The ND50 is typically0.15‑0.6 µg/mL.


目次に戻る

カートに商品を
追加しました。

Related Product & Information

Entrez Gene IDs941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
BackgroundB7-1/CD80
background_contentBackground:
B7-1/CD80
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in thedown‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouseB7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.


目次に戻る

カートに商品を
追加しました。

Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
  1. High fat diet and leptin promote tumor progression by inducing myeloid-derived suppressor cells
    Authors: VK Clements, T Long, R Long, C Figley, DMC Smith, S Ostrand-Ro
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

  2. Aging is associated with increased regulatory T-cell function.
    Authors: Garg S, Delaney C, Toubai T, Ghosh A, Reddy P, Banerjee R, Yung R
    Aging Cell, 2014;13(3):441-8.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

  3. Antigen-loaded ER microsomes from APC induce potent immune responses against viral infection.
    Authors: Sofra V, Mansour S, Liu M, Gao B, Primpidou E, Wang P, Li S
    Eur. J. Immunol., 2009;39(1):85-95.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB

  4. Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
    Authors: He H, Li W, Chen SY, Zhang S, Chen YT, Hayashida Y, Zhu YT, Tseng SC
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4468-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC



目次に戻る

カートに商品を
追加しました。

お問い合わせ先

（テクニカルサポート 試薬担当）

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。