抗IFN-γ抗体(Anti-IFN-γ, Rat, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:27134

IFN-γに対する抗体(Anti-IFN-γ, Rat, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2025年07月01日 12時15分現在]

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Anti-IFN-γ, Rat, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 8
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説明文
別名:IFG
Genbank No: 3458
Protein Accession No: P01581
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Rat 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事
Anti-Mouse/Rat IFN-gamma Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
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説明文
※受注発注品。形状:溶液または凍結乾燥
別名:IFG
Genbank No: 3458
Protein Accession No: P01581
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 IC,Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事

[在庫・価格 :2025年07月01日 12時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-IFN-γ, Rat, Goat-Poly

文献数: 8

説明文 別名:IFG
Genbank No: 3458
Protein Accession No: P01581
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Rat 免疫動物 Goat
交差性 Mouse 適用 IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事

Anti-Mouse/Rat IFN-gamma Affinity Purified Polyclonal Ab

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:IFG
Genbank No: 3458
Protein Accession No: P01581
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Mouse 適用 IC,Neutralising,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
使いっきり抗体
関連記事



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Product Details

Species ReactivityMouse, Rat
LabelUnconjugated
ImmunogenE. coli-derived recombinant rat IFN-gamma Gln23-Cys156Accession # P01581
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects rat and mouse IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IFN-gamma, recombinant canine IFN-gamma, recombinant feline IFN‑ gamma, recombinant equine IFN-gamma, recombinant porcine IFN-gamma, recombinant rhesus monkey IFN-gamma, recombinant cotton rat IFN-gamma, and recombinant bovine IFN‑ gamma is observed. In Western blots, less than 10% cross‑reactivity with rhIFN‑ gamma is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot0.1 µg/mLRecombinant Rat IFN-gamma (Catalog # 585-IF)
Immunocytochemistry5-15 µg/mLSee below
NeutralizationMeasured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.08-0.2 µg/mL in the presence of 2.5 ng/mL Recombinant Rat IFN‑ gamma.


Neutralization
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse/Rat IFN‑ gamma  Antibody.
Recombinant Rat IFN‑ gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND50 is typically0.08‑0.2 µg/mL.
Immunocytochemistry
IFN‑ gamma in Rat Splenocytes.
IFN‑ gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.


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Related Product & Information

Long NameInterferon gamma
Entrez Gene IDs3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
BackgroundIFN-gamma
background_contentBackground:
IFN-gamma
Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37-45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
    Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
    Sci Rep, 2017;7(0):46514.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

  2. Expression of pro-inflammatory cytokines in the auditory cortex of rats with salicylate-induced tinnitus
    Authors: XH Chen, LL Zheng
    Mol Med Rep, 2017;0(0):.

  3. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
    Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
    J Neurosci, 2014;34(6):2231-43.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut

  4. Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.
    Authors: Rana S, Byrne SN, MacDonald LJ, Chan CY, Halliday GM
    Am. J. Pathol., 2008;172(4):993-1004.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen



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