抗VEGF抗体(Anti-VEGF, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant rat VEGF164Ala27-Arg190Accession # AAL07526
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF165 and rhVEGF121 is observed and less than 2% cross-reactivity with rhVEGF-B, recombinant mouse (rm) VEGF-B, rhVEGF-C, rhVEGF-D, and rmVEGF-D is observed. In Western blots, detection of recombinant mouse VEGF165 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 20 ng/mL Recombinant Rat VEGF164.

Immunohistochemistry
	VEGF164 in Rat Kidney. VEGF164 was detected in perfusion fixed frozen sections of rat kidney using 15 µg/mL Goat Anti-Rat VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	Cell Proliferation Induced by VEGF164 and Neutralization by Rat VEGF Antibody. Recombinant Rat VEGF164 (Catalog # 564-RV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat VEGF164 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564). The ND50 is typically 0.2-0.6 µg/mL.
Western Blot
	Detection of Recombinant Rat and Mouse VEGF by Western Blot. Western blot shows 25 ng of Recombinant Rat VEGF 164 (Catalog # 564-RV), Recombinant Human VEGF 165 (Catalog # 293-VE) and Recombinant Mouse VEGF 164 (Catalog # 493-MV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 20-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.This antibody does not detect natural VEGF in lysates from cell lines or tissues.

目次に戻る

Related Product & Information

Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Background	VEGF
background_content	Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues. Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164, and 188 aa isoforms found in rat and mouse. Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible. Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the semaphorin receptor, neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M, and TNF-alpha.

目次に戻る

Citations

1. Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels
   Authors: L Melly, G Cerino, A Frobert, S Cook, MN Giraud, T Carrel, HT Tevaearai, F Eckstein, B Rondelet, A Marsano, A Banfi
   J. Cell. Mol. Med., 2018;0(0):.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC-F


2. Coronary angiogenic effect of long-term administration of Nigella sativa
   Authors: LI Al Asoom
   BMC Complement Altern Med, 2017;17(1):308.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


3. Genome-wide expression differences in anti-Vegf and dexamethasone treatment of inflammatory angiogenesis in the rat cornea
   Authors: P Mirabelli, A Mukwaya, A Lennikov, M Xeroudaki, B Peebo, M Schaupper, N Lagali
   Sci Rep, 2017;7(1):7616.
   Species: Rat
   Sample Type: In Vivo
   Application: Neut


4. Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
   Authors: Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M
   PLoS ONE, 2015;10(3):e0120235.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Not Specified


5. FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
   Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J
   PLoS ONE, 2015;10(7):e0134392.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity.
   Authors: Wang H, Yang Z, Jiang Y, Flannery J, Hammond S, Kafri T, Vemuri S, Jones B, Hartnett M
   Invest Ophthalmol Vis Sci, 2014;55(2):737-44.
   Species: Rat
   Sample Type: In Vivo
   Application: In vivo


7. The probiotic mixture VSL#3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor.
   Authors: Dharmani P, De Simone C, Chadee K
   PLoS ONE, 2013;8(3):e58671.
   Species: Rat
   Sample Type: Whole Tissue
   Application: Neut


8. Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy.
   Authors: Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
   PLoS ONE, 2013;8(12):e83846.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


9. Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity.
   Authors: McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M
   Invest Ophthalmol Vis Sci, 2013;54(3):2020-6.
   Species: Rat
   Sample Type: In Vivo
   Application: In vivo


10. Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
    Authors: Westenbrink BD, Ruifrok WP, Voors AA
    Cardiovasc. Res., 2010;87(1):30-9.
    Species: Rat
    Sample Type: In Vivo
    Application: In vivo

目次に戻る