抗ST2抗体(Anti-ST2, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 RLys19-Phe328Accession # BAA02233
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) ST2/IL-33 R is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC). Blom, L and Poulsen, LK (2012) JI 189:4331. The Neutralization Dose (ND50) is typically 0.1-0.6 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.

Western Blot
	Detection of Human ST2/IL-33 R by Western Blot. Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-HumanST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ST2/IL-33 R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of ST2/IL-33 R in KG‑1a Human Cell Line by Flow Cytometry. KG‑1a human acute myelogenous leukemia cell line was stained with Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Simple Western
	Detection of Human ST2/IL-33 R by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ST2/IL-33 R at approximately 60 kDa (as indicated) using 2 µg/mL of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization
	IFN-gamma Secretion Induced byIL‑33 and Neutralization by Human ST2/IL-33 R Antibody. Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of 0.25 ng/mL Recombinant Human IL-12 (Catalog # 219-IL)  in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Goat Anti-Human ST2/IL-33 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF523). The ND50 is typically 0.1-0.6 μg/mL.

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Related Product & Information

Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Background	ST2/IL-33 R
background_content	Background: ST2/IL-33 R ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33’s ability to signal through transmembrane ST2 (10, 13‑15).

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Citations

1. IL-33 stimulates CXCL8/IL-8 secretion in goblet cells but not normally differentiated airway cells.
   Authors: Tanabe T, Shimokawaji T, Kanoh S, Rubin B
   Clin Exp Allergy, 2014;44(4):540-52.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Detection of soluble ST2 in human follicular fluid and luteinized granulosa cells.
   Authors: Southcombe J, Ledee N, Perrier d'Hauterive S, Turner K, Child T, Snider J, Redman C, Sargent I, Granne I
   PLoS ONE, 2013;8(9):e74385.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. ST2 and IL-33 in pregnancy and pre-eclampsia.
   Authors: Granne I, Southcombe JH, Snider JV
   PLoS ONE, 2011;6(9):e24463.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts.
   Authors: Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y
   Gut, 2010;59(4):531-41.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils.
   Authors: Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H
   J. Allergy Clin. Immunol., 2008;121(6):1484-90.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Gene induction during differentiation of human pulmonary type II cells in vitro.
   Authors: Wade KC, Guttentag SH, Gonzales LW, Maschhoff KL, Gonzales J, Kolla V, Singhal S, Ballard PL
   Am. J. Respir. Cell Mol. Biol., 2006;34(6):727-37.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


7. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines.
   Authors: Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA
   Immunity, 2005;23(5):479-90.
   Species: Human
   Sample Type: Recombinant Protein
   Application: WB


8. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
   Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
   J Korean Med Sci, 0;26(9):1132-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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