抗IL-6抗体(Anti-IL-6, Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat IL-6Phe25-Thr211Accession # P20607
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat and mouse IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant cotton rat IL‑6 is observed, and less than 1% cross-reactivity with recombinant human IL-6 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.6 ng/mL Recombinant Rat IL‑6.

Western Blot
	Detection of Rat IL‑6 by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line  untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-RatIL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	IL‑6 in Mouse T Cells. IL‑6 was detected in immersion fixed activated mouse T Cells using 15 µg/mL Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
	Cell Proliferation Induced by IL‑6 and Neutralization by Rat IL‑6 Antibody. Recombinant Rat IL‑6 (Catalog # 506-RL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant RatIL‑6 (0.6 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF506). The ND50 is typically 0.1-0.5 µg/mL.

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Related Product & Information

Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
Background	IL-6
background_content	Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature rat IL-6 is 187 amino acids (aa) in length and shares 41% and 85% aa sequence identity with human and mouse IL-6, respectively (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surfaceIL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

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Citations

1. Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-? oligomers
   Authors: MA Godoy, LM Saraiva, LRP Carvalho, A Vasconcelo, HJV Beiral, AB Ramos, LRP Silva, RB Leal, VHS Monteiro, CV Braga, CA Araujo-Sil, LC Sinis, VB Santos, THK Brunswick, CL Alcantara, APCA Lima, NLD Cunha E Si, A Galina, A Vieyra, FG De Felice, R Mendez-Ote, ST Ferreira
   J. Biol. Chem., 2017;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


2. Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcohol
   Authors: P Shrivastav, MA Cabrera, LG Chastain, NI Boyadjieva, S Jabbar, T Franklin, DK Sarkar
   J Neuroinflammation, 2017;14(1):83.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


3. Repeated water avoidance stress induces visceral hypersensitivity; role of IL-1, IL-6 and peripheral corticotropin-releasing factor
   Authors: T Nozu, S Miyagishi, R Nozu, K Takakusaki, T Okumura
   J. Gastroenterol. Hepatol, 2017;0(0):.
   Species: Rat
   Sample Type: In Vivo
   Application: In Vivo


4. Sublytic C5b-9 induces IL-6 and TGF-beta1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPbeta acetylation.
   Authors: Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y
   FASEB J, 2014;28(3):1511-25.
   Species: Rat
   Sample Type: Whole Cells
   Application: Bioassay


5. Electrical stimulation induces IL-6 in skeletal muscle through extracellular ATP by activating Ca(2+) signals and an IL-6 autocrine loop.
   Authors: Bustamante M, Fernandez-Verdejo R, Jaimovich E, Buvinic S
   Am J Physiol Endocrinol Metab, 2014;306(8):E869-82.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


6. Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
   Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
   J Neurosci, 2014;34(6):2231-43.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


7. Mechanisms involved in dual vasopressin/apelin neuron dysfunction during aging.
   Authors: Sauvant J, Delpech J, Palin K, De Mota N, Dudit J, Aubert A, Orcel H, Roux P, Laye S, Moos F, Llorens-Cortes C, Nadjar A
   PLoS ONE, 2014;9(2):e87421.
   Species: Rat
   Sample Type: In Vivo
   Application: Neut


8. Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
   Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
   J Am Soc Nephrol, 2013;24(4):587-603.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


9. Fetal asphyctic preconditioning modulates the acute cytokine response thereby protecting against perinatal asphyxia in neonatal rats.
   Authors: Vlassaks E, Strackx E, Vles J, Nikiforou M, Martinez-Martinez P, Kramer B, Gavilanes A
   J Neuroinflammation, 2013;10(0):14.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus.
    Authors: Oh J, McCloskey MA, Blong CC
    J. Neurosci. Res., 2010;88(13):2798-809.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut

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