抗Flt-1抗体(Anti-Flt-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt-1Ser27-Glu759Accession # P35969
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VEGF R1/Flt-1 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant human VEGF R1 is observed, approximately 4% cross-reactivity with recombinant mouse (rm) VEGF164 is observed, less than 1% cross-reactivity with recombinant rat VEGF164 is observed, and less than 0.2% cross-reactivity with rmVEGF R2 and rmVEGF R3 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse VEGF R1/Flt‑1 Fc Chimera (Catalog # 471-F1)
Flow Cytometry	2.5 µg/106 cells	bEnd.3 mouse endothelioma cell line
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse PlGF-2 (Catalog # 465-PL) to immobilized Recombinant Mouse VEGF R1/Flt-1 Fc Chimera (Catalog # 471-F1) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Mouse VEGF R1/Flt-1 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse VEGF R1/Flt‑1 Antibody (Catalog #AF471 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse VEGF R1/Flt‑1 Biotinylated Antibody (Catalog #BAF471 )
ELISA Standard		Recombinant Mouse VEGF R1/Flt-1 Fc Chimera Protein, CF (Catalog #471-F1 )
Neutralization	Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 5 ng/mL Recombinant Mouse VEGF164.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF R1/Flt‑1 Antibody.Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND50 is typically 2-8 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGF R1/Flt-1	VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

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References

He, Y. et al. (1999) Molecular Endocrinology 13:537.
Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.
Long Name:	Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs:	2321 (Human); 14254 (Mouse)
Alternate Names:	EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Related Research Areas

Airway Inflammation and Remodeling

Cancer Biomarkers

Cytokine and Growth Factor Receptors on VSMC

Endothelial Cell Differentiation Markers

Endothelial Cell Markers

Endothelial Progenitor and Endothelial Cell Markers

HIF Transcription Factors

Myeloid-derived Suppressor Cells (MDSC)

Preeclampsia

Receptor Tyrosine Kinases (RTKs)

Receptor Tyrosine Kinases (RTKs) in the Akt Pathway

Receptors in the Jak/STAT Pathway

VEGF Family

VEGF Receptors and Regulators on Endothelial Cells

Mechanistic Insights into the Anti-angiogenic Activity of Trypanosoma cruzi Protein 21 and its Potential Impact on the Onset of Chagasic Cardiomyopathy Authors: SC Teixeira, DS Lopes, SN Gimenes, TL Teixeira, MS da Silva, RT Brígido, FA da Luz, AA da Silva, MA Silva, PV Florentino, PC Tavares, MA Dos Santos, VM Ávila, MJ Silva, MC Elias, RA Mortara, CV da Silva Sci Rep, 2017;7(0):44978. Species: Mouse Sample Type: Cell Lysates Application: WB

Neutralization

Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF R1/Flt‑1 Antibody. Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND50 is typically 2-8 µg/mL.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
Background	VEGF R1/Flt-1
background_content	Background: VEGF R1/Flt-1 VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis. Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

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Citations

1. Mechanistic Insights into the Anti-angiogenic Activity of Trypanosoma cruzi Protein 21 and its Potential Impact on the Onset of Chagasic Cardiomyopathy
   Authors: SC Teixeira, DS Lopes, SN Gimenes, TL Teixeira, MS da Silva, RT Brígido, FA da Luz, AA da Silva, MA Silva, PV Florentino, PC Tavares, MA Dos Santos, VM Ávila, MJ Silva, MC Elias, RA Mortara, CV da Silva
   Sci Rep, 2017;7(0):44978.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
   Nat Commun, 2016;7(0):13650.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage.
   Authors: Hou, Sheng Ta, Nilchi, Ladan, Li, Xuesheng, Gangaraju, Sandhya, Jiang, Susan X, Aylsworth, Amy, Monette, Robert, Slinn, Jacqueli
   Sci Rep, 2015;5(0):7890.
   Species: Rat
   Sample Type: Whole Cells
   Application: Bioassay


4. Differential apicobasal VEGF signaling at vascular blood-neural barriers.
   Authors: Hudson N, Powner M, Sarker M, Burgoyne T, Campbell M, Ockrim Z, Martinelli R, Futter C, Grant M, Fraser P, Shima D, Greenwood J, Turowski P
   Dev Cell, 2014;30(5):541-52.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. The role of IL-1beta in the early tumor cell-induced angiogenic response.
   Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
   J Immunol, 2013;190(7):3500-9.
   


6. Nonmuscle myosin light-chain kinase mediates microglial migration induced by HIV Tat: involvement of beta1 integrins.
   Authors: Yao H, Duan M, Yang L, Buch S
   FASEB J, 2013;27(4):1532-48.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


7. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
   Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
   Nature, 2012;484(7392):110-4.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: IP


8. The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy.
   Authors: Schreurs MP, Houston EM, May V, Cipolla MJ
   FASEB J., 2012;26(1):355-62.
   Species: Rat
   Sample Type: In Vivo
   Application: In vivo


9. Hypoxia-inducible factor-2alpha regulates GM-CSF-derived soluble vascular endothelial growth factor receptor 1 production from macrophages and inhibits tumor growth and angiogenesis.
   Authors: Roda JM, Sumner LA, Evans R, Phillips GS, Marsh CB, Eubank TD
   J. Immunol., 2011;187(4):1970-6.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


10. Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells.
    Authors: Shin JY, Yoon IH, Kim JS, Kim B, Park CG
    Cell. Immunol., 2009;256(1):72-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

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