抗VEGF-D抗体(Anti-VEGF-D, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse VEGF-DPhe98-Ser206Accession # P97946
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VEGF-D in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) VEGF-D is observed, and less than 1% cross-reactivity with recombinant mouse (rm) VEGF115, rmVEGF120, rhVEGF, rhVEGF-B, and rmVEGF165 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse VEGF-D (Catalog # 469-VD)
Immunohistochemistry	5-15 µg/mL	See below

Immunohistochemistry

VEGF-D in Mouse Embryo. VEGF-D was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse VEGF‑D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to seminiferous tubules in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

VEGF-D

background_content

Background: VEGF-D Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1-4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF‑D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14).

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Citations

1. Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.
   Authors: Donoghue JF, McGavigan CJ, Lederman FL
   PLoS ONE, 2012;7(2):e30916.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model.
   Authors: Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ
   Int. J. Cancer, 2009;124(11):2709-18.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


3. Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
   Authors: Kivela R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
   Am. J. Physiol. Heart Circ. Physiol., 2007;293(4):H2573-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh


4. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
   Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
   Nat. Neurosci., 2006;9(3):340-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen

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