抗Fas抗体(Anti-Fas, Mouse, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：27004
Fasに対する抗体(Anti-Fas, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年05月18日 00時00分現在]
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Anti-Fas, Mouse, Goat-Poly <Anti-CD95> |
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Anti-Mouse Fas/TNFRSF6/CD95 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年05月18日 00時00分現在]
Anti-Fas, Mouse, Goat-Poly <Anti-CD95>
文献数： 5
- 商品コード：AF435
- メーカー：RSD
- 包装：100μg
- 価格：¥105,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse Fas/TNFRSF6/CD95 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF435，スタンダードとして#435-FA-050を用いる。 別名：Apo-1 Genbank No： 355 Protein Accession No： P25446 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Goat | ||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse Fas/TNFRSF6/CD95 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF435-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：Apo-1 Genbank No： 355 Protein Accession No： P25446 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Fas/TNFRSF6/CD95Gly14-Arg169Accession # P25446 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse Fas/TNFRSF6/CD95 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross‑reactivity with recombinant human (rh) Fas, rh4-1BB, rhDR6, rhNGF R, recombinant mouse (rm) OPG, rmRANK and rmCD30 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 435-FA) |
| Flow Cytometry | 2.5 µg/106 cells | Mouse splenocytes |
| Immunohistochemistry | 5-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Mouse Fas/TNFRSF6/CD95 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse Fas/TNFRSF6/CD95 Antibody (Catalog #AF435 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse Fas/TNFRSF6/CD95 Biotinylated Antibody (Catalog #BAF435 ) |
| ELISA Standard | Recombinant Mouse Fas/TNFRSF6/CD95 Fc Chimera Protein (Catalog #435-FA ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunohistochemistry |
 click image to view larger | Fas/TNFRSF6/CD95 in Mouse Embryo.Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Fas/TNFRSF6/CD95 | Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas. Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells. |
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References
| Nagata, S. and P. Golstein (1995) Science 267:1449. | |
| Nagata, S. (1997) Cell 88:355. | |
| Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355. | |
| Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA. 94:5986. | |
| Long Name: | Fibroblast-associated |
| Entrez Gene IDs: | 355 (Human); 14102 (Mouse); 246097 (Rat); 493881 (Feline) |
| Alternate Names: | Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6 |
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Related Research Areas
| Adipocytokines | ||
| Alpha Granule Soluble Molecules | ||
| B Cell Development and Differentiation | ||
| Cytokine and Growth Factor Receptors on VSMC | ||
| gamma delta T Cells | ||
| Immune Regulatory Molecules on Endothelial Cells | ||
| Isolation, Culture, and Identification of Natural Killer Cells | ||
| Leukocyte Receptors/Markers in Tfh Cell Differentiation and Function | ||
| Regulation of Apoptosis by TNF Superfamily Members | ||
| Regulatory T Cells (Tregs) | ||
| TNF Superfamily | ||
| TNF Superfamily Receptors | ||
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| Immunohistochemistry | |
|---|---|
 | Fas/TNFRSF6/CD95 in Mouse Embryo. Fas/TNFRSF6/CD95 was detected in immersion fixed frozen sections of mouse embryo (E15) using Goat Anti-Mouse Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF435) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to olfactory epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
追加しました。
Related Product & Information
| Background | Fas/TNFRSF6/CD95 |
|---|---|
| background_content | Background: Fas/TNFRSF6/CD95 Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human and mouse Fas cDNAs encode a 325 and a 327 amino acid residue type 1 membrane protein, respectively, that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple human Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas. Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells. |
追加しました。
Citations
- Induced autoimmunity to heat shock proteins elicits glaucomatous loss of retinal ganglion cell neurons via activated T-cell-derived fas-ligand.
Authors: Wax MB, Tezel G, Yang J, Peng G, Patil RV, Agarwal N, Sappington RM, Calkins DJ
J. Neurosci., 2008;28(46):12085-96.
Species: Rat
Sample Type: Whole Tissue
Application: IHC - Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
Am. J. Respir. Cell Mol. Biol., 2005;33(3):248-53.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
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