抗IL-4抗体(Anti-IL-4, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-4His23-Ser140Accession # P07750
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects mouse IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL‑4 and no cross-reactivity with recombinant human IL-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑4 (Catalog # 404-ML)
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 3-5 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.

Neutralization

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody. Recombinant Mouse IL‑4 (Catalog # 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA). The ND50 is typically 3-5 µg/mL.

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Related Product & Information

Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Background	IL-4
background_content	Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11-14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

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Citations

1. The spleen is the site where mast cells are induced in the development of food allergy
   Authors: S Toyoshima, E Wakamatsu, Y Ishida, Y Obata, Y Kurashima, H Kiyono, R Abe
   Int. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
   Authors: El-Behi M, Ciric B, Dai H
   Nat. Immunol., 2011;12(6):568-75.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
   Authors: Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, Yu S, Saris CJ, Gran B, Ciric B, Rostami A
   Nat. Immunol., 2007;8(12):1372-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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