抗CD4抗体(Anti-CD4, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4Lys26-Trp390Accession # P01730
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4 and recombinant rat CD4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CD4 (Catalog # 514-CD)
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human tonsil
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	CD4 in Human T Cells. CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

CD4

background_content

Background: CD4 CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

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Citations

1. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis.
   Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P
   J Clin Invest, 2015;125(2):739-51.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


2. HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells.
   Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S
   J Virol, 2014;88(19):11045-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo.
   Authors: Suzuki, Yasuhiro, Gatanaga, Hiroyuki, Tachikawa, Natsuo, Oka, Shinichi
   PLoS ONE, 2014;9(2):e86479.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
   Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
   J Allergy Clin Immunol, 2013;131(4):1117-29, 1129.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


5. A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages.
   Authors: Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J
   PLoS Pathog, 2012;8(9):e1002937.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells.
   Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V
   J. Immunol., 2009;183(7):4792-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies.
   Authors: Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE
   Arthritis Rheum., 2007;56(1):372-83.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


8. Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress.
   Authors: Bjerke SL, Roller RJ
   Virology, 2006;347(2):261-76.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
   Authors: Wang B, He J, Liu C, Chang LJ
   Vaccine, 2006;24(17):3477-89.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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