抗TARC抗体(Anti-TARC, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL17/TARC (R&D Systems, Catalog # 364-DN)Ala24-Ser94Accession # Q92583
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CCL17/TARC (Catalog # 364-DN)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL17/TARC.

Neutralization
	Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog #364‑DN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/TARC (0.01 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND50 is typically 0.3-1.5 µg/mL.
Immunohistochemistry
	CCL17/TARC in Human Spleen. CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	6361 (Human); 20295 (Mouse)
Background	CCL17/TARC
background_content	Background: CCL17/TARC CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

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Citations

1. miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis
   Authors: Y Yuan, J Kluiver, J Koerts, D de Jong, B Rutgers, FRA Razak, M Terpstra, B Plaat, IM Nolte, A Diepstra, L Visser, K Kok, A van den Be
   Am. J. Pathol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
   Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
   J. Immunol., 2010;184(6):2886-98.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
   Authors: Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
   Thorax, 2008;63(9):810-6.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
   Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
   J. Immunol., 2007;178(12):7678-85.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
   Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
   Blood, 2007;111(4):2339-46.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
   Authors: Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, Van Den Berg A
   J. Pathol., 2006;209(4):474-83.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
   Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
   Int. J. Oncol., 2004;25(3):605-13.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. TARC augments TNF-alpha-induced CTACK production in keratinocytes.
   Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
   Exp. Dermatol., 2004;13(9):551-7.
   Species: Human
   Sample Type: Cell Culture Supernates
   


9. Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
   Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
   Int. J. Cancer, 2002;99(5):665-72.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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