抗SCF Receptor抗体(Anti-SCF Receptor, Human, Goat-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：26921
SCF Receptorに対する抗体(Anti-SCF Receptor, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年04月26日 08時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-SCF Receptor, Human, Goat-Poly <Anti-CD117> |
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Anti-Human CD117/c-kit Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年04月26日 08時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-SCF Receptor, Human, Goat-Poly <Anti-CD117>
文献数： 7
- 商品コード：AF332
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：CD117 Genbank No： 3815 Protein Accession No： P10721 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | FCM,IHC,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体 抗間葉系幹細胞マーカー抗体 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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Anti-Human CD117/c-kit Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF332-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD117 Genbank No： 3815 Protein Accession No： P10721 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | FCM,IHC,Neutralising,Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kitGln26-Thr520Accession # P10721 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human PDGF sR alpha is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | See below |
| Simple Western | 5 µg/mL | See below |
| Flow Cytometry | 2.5 µg/106 cells | TF‑1 human erythroleukemic cell line |
| Immunohistochemistry | 5-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Neutralization | Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The Neutralization Dose (ND50) is typically 0.06-0.36 μg/mL in the presence of 20 ng/mL Recombinant Human SCF/c‑kit Ligand. | |
| Neutralization | |
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 | Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human CD117/c-kit Antibody. Recombinant Human SCF/c‑kit Ligand (Catalog # 255-SC) induces cell proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited bySCF/c‑kit Ligand is neutralized (green line) by increasing concentrations of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332). The ND50 is typically 0.06-0.36 μg/mL in the presence of 20 ng/mL of Recombinant HumanSCF/c‑kit Ligand. |
| Immunohistochemistry | |
 | CD117/c-kit in Human Brain. CD117/c-kit was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
| Western Blot | |
 | Detection of Human CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD117/c-kit at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Simple Western | |
 | Detection of Human CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 5 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
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Related Product & Information
| Long Name | Stem Cell Factor Receptor |
|---|---|
| Entrez Gene IDs | 3815 (Human); 16590 (Mouse); 64030 (Rat) |
| Background | CD117/c-kit |
| background_content | Background: CD117/c-kit Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
J. Invest. Dermatol., 2010;130(6):1685-96.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Chemotherapy-induced thrombocytopenia derives from the selective death of megakaryocyte progenitors and can be rescued by stem cell factor.
Authors: Zeuner A, Signore M, Martinetti D, Bartucci M, Peschle C, De Maria R
Cancer Res., 2007;67(10):4767-73.
Species: Human
Sample Type: Whole Cells
Application: ICC - Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cells.
Authors: Levesque JP, Hendy J, Winkler IG, Takamatsu Y, Simmons PJ
Exp. Hematol., 2003;31(2):109-17.
Species: Human
Sample Type: Recombinant Protein
Application: WB
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