抗Flt-1抗体(Anti-Flt-1, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1Ser27-His687Accession # NP_001153392
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant mouse VEGF R1 and less than 10% cross-reactivity with recombinant human (rh) VEGF R2 and rhVEGF R3.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human VEGF R1/Flt‑1 Fc Chimera (Catalog # 321-FL)
Flow Cytometry	2.5 µg/106 cells	HUVEC human umbilical vein endothelial cells
Immunohistochemistry	5-15 µg/mL	See below
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human PlGF (Catalog # 264-PG) to immobilized Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 321-FL) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry
	VEGF R1/Flt‑1 in Human Breast Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	VEGF R1/Flt‑1 in Human Ovarian Cancer Tissue. VEGF R1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGF R1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs	2321 (Human); 14254 (Mouse)
Background	VEGF R1/Flt-1
background_content	Background: VEGF R1/Flt-1 Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

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Citations

1. VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration process
   Authors: S Capossela, A Bertolo, K Gunasekera, T Pötzel, M Baur, JV Stoyanov
   BMC Res Notes, 2018;11(1):333.
   Species: Bovine
   Sample Type: Cell Lysates
   Application: WB


2. Placental soluble fms-like tyrosine kinase expression in small for gestational age infants and risk for adverse outcomes
   Authors: M Spiel, S Salahuddin, E Pernicone, Z Zsengeller, A Wang, AM Modest, SA Karumanchi, JL Hecht
   Placenta, 2017;52(0):10-16.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


3. Ferrochelatase is a therapeutic target for ocular neovascularization
   Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
   EMBO Mol Med, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis.
   Authors: Kim H, Kim K, Kim B, Cho M, Lee S
   PLoS ONE, 2015;10(4):e0124909.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration.
   Authors: Chanakira A, Kir D, Barke R, Santilli S, Ramakrishnan S, Roy S
   PLoS ONE, 2015;10(9):e0138587.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice.
   Authors: Szalai G, Romero R, Chaiworapongsa T, Xu Y, Wang B, Ahn H, Xu Z, Chiang P, Sundell B, Wang R, Jiang Y, Plazyo O, Olive M, Tarca A, Dong Z, Qureshi F, Papp Z, Hassan S, Hernandez-Andrade E, Than N
   PLoS ONE, 2015;10(4):e0119547.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Melanoma spheroid formation involves laminin-associated vasculogenic mimicry.
   Authors: Larson A, Lee C, Lezcano C, Zhan Q, Huang J, Fischer A, Murphy G
   Am J Pathol, 2014;184(1):71-8.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC


8. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
   Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
   Mol Ther, 2014;22(7):1243-53.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


9. Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
   Authors: Hou H, Cheng S, Chung K, Kuo M, Yeh C, Chang B, Lu H, Wang H, Yu C
   Autophagy, 2014;10(9):1509-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts.
    Authors: Lezcano C, Kleffel S, Lee N, Larson A, Zhan Q, DoRosario A, Wang L, Schatton T, Murphy G
    Lab Invest, 2014;94(10):1092-102.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen

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