抗IL-15抗体(Anti-IL-15, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑15 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑15 (Catalog # 247-IL)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line [Avanzi, G. et al. (1988) Br. J. Haematol. 69:359]. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 10 ng/mL of Recombinant Human IL‑15.

Neutralization
	Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (10 ) is neutralized (green line) by increasing concen­trations of Goat Anti-Human IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315). The ND50 is typically 0.5-1.5 µg/mL.
Immunohistochemistry
	IL‑15 in Human Tonsil. IL‑15 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Background	IL-15
background_content	Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. A First-in-Human Phase 1 Study of Subcutaneous Outpatient Recombinant Human IL-15 (rhIL-15) in Adults with Advanced Solid Tumors
   Authors: JS Miller, C Morishima, DG McNeel, MR Patel, HE Kohrt, JA Thompson, PM Sondel, HA Wakelee, ML Disis, JC Kaiser, MA Cheever, H Streicher, SP Creekmore, TA Waldmann, KC Conlon
   Clin. Cancer Res., 2017;0(0):.
   Application: Standard


2. IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis.
   Authors: Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N
   J Immunol, 2015;194(5):2099-109.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC- Frozen


3. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
   Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
   Gene Ther, 2015;22(1):76-86.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells.
   Authors: Mongini P, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz J, Allen S, Rai K, Chu C, Chiorazzi N
   J Immunol, 2015;195(3):901-23.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
   Authors: Marcondes MC, Burdo TH, Sopper S, Huitron-Resendiz S, Lanigan C, Watry D, Flynn C, Zandonatti M, Fox HS
   J. Immunol., 2007;178(9):5812-9.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Cloning and expression of feline interleukin 15.
   Authors: Dean GA, Barger A, Lavoy A
   Cytokine, 2005;29(2):77-83.
   Species: Feline
   Sample Type: Whole Cells
   Application: ICC


7. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
   Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
   J. Immunol., 2005;174(4):2376-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
   Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
   J. Immunol., 2004;172(1):130-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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