抗VEGF抗体(Anti-VEGF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165Ala27-Arg191Accession # AAV38412
Source	Polyclonal Goat IgG
Purification	Protein A or G purified
Specificity	Detects human VEGF165 and human VEGF121 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VEGF and recombinant rat VEGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human VEGF165 (Catalog # 293-VE)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically 0.6‑3.0 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Neutralization
	Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human VEGF 165 Polyclonal Antibody (Catalog # AB-293-NA). The ND50 is typically 0.6-3.0 µg/mL.
Immunohistochemistry
	VEGF165 in Human Breast Cancer Tissue. VEGF165 was detected in immersion fixed frozen sections of human breast cancer tissue using 5 µg/mL Human VEGF165 Polyclonal Antibody (Catalog # AB‑293‑NA) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	VEGF in Human Breast Cancer Tissue. VEGF was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human VEGF 165 Polyclonal Antibody (Catalog # AB-293-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Background	VEGF
background_content	Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1‑3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

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Citations

1. High Plasma Lipid Levels Reduce Efficacy of Adenovirus-Mediated Gene Therapy
   Authors: AM Kivelä, J Huusko, E Gurzeler, A Laine, MH Dijkstra, G Dragneva, CB Andersen, SK Moestrup, S Ylä-Herttu
   Sci Rep, 2017;7(1):386.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. A phase Ib/II translational study of sunitinib with neoadjuvant radiotherapy in soft-tissue sarcoma.
   Authors: Lewin J, Khamly K, Young R, Mitchell C, Hicks R, Toner G, Ngan S, Chander S, Powell G, Herschtal A, Te Marvelde L, Desai J, Choong P, Stacker S, Achen M, Ferris N, Fox S, Slavin J, Thomas D
   Br J Cancer, 2014;111(12):2254-61.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma.
   Authors: Kondo S, Mukudai Y, Soga D, Nishida T, Takigawa M, Shirota T
   Anticancer Res, 2014;34(2):671-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Extensive podocyte loss triggers a rapid parietal epithelial cell response.
   Authors: Hakroush S, Cebulla A, Schaldecker T, Behr D, Mundel P, Weins A
   J Am Soc Nephrol, 2014;25(5):927-38.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesis.
   Authors: Uniewicz KA, Cross MJ, Fernig DG
   J. Biol. Chem., 2011;286(1):12-23.
   Species: Human
   Sample Type: Buffer
   Application: Other: See notes


6. Vascular endothelial growth factor is up-regulated after status epilepticus and protects against seizure-induced neuronal loss in hippocampus.
   Authors: Nicoletti JN, Shah SK, McCloskey DP, Goodman JH, Elkady A, Atassi H, Hylton D, Rudge JS, Scharfman HE, Croll SD
   Neuroscience, 2007;151(1):232-41.
   Species: Rat
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. Vascular endothelial growth factor (VEGF) expression in oral tissues: possible relevance to angiogenesis, tumour progression and field cancerisation.
   Authors: Carlile J, Harada K, Baillie R, Macluskey M, Chisholm DM, Ogden GR, Schor SL, Schor AM
   J. Oral Pathol. Med., 2001;30(8):449-57.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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