抗TNF-alpha抗体(Anti-TNF-α, Human, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26821

TNF-alphaに対する抗体(Anti-TNF-α, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2026年04月06日 18時15分現在]

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Anti-TNF-α, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 6
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説明文
別名:APC1 protein
Genbank No: 7124
Protein Accession No: P01375
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事

[在庫・価格 :2026年04月06日 18時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-TNF-α, Human, Goat-Poly

文献数: 6

説明文 別名:APC1 protein
Genbank No: 7124
Protein Accession No: P01375
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
Helper T Cells Marker Antibodies
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenE. coli-derived recombinant human TNF-alpha Val77-Leu233Accession # P01375
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant canine TNF‑ alpha, recombinant bovine TNF‑ alpha, recombinant rat TNF‑ alpha, recombinant equine TNF‑ alpha, and recombinant cotton rat TNF‑ alpha is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Human TNF‑ alpha (Catalog # 210-TA)
NeutralizationMeasured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221]. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ alpha and 1 µg/mL actinomycin D.


Neutralization
Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody.
Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human TNF‑ alpha Polyclonal Antibody (Catalog # AB-210-NA). The ND50 is typically 0.05-0.25 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).


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Related Product & Information

Long NameTumor Necrosis Factor alpha
Entrez Gene IDs7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
BackgroundTNF-alpha
background_contentBackground:
TNF-alpha
Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
    Authors: Liang X, Moseman EA, Farrar MA
    Blood, 2010;115(24):5041-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

  2. Antigenic stimulation in the simian model of HIV infection yields dilated cardiomyopathy through effects of TNFalpha.
    Authors: Yearley JH, Mansfield KG, Carville AA, Sokos GG, Xia D, Pearson CB, Shannon RP
    AIDS, 2008;22(5):585-94.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB

  3. Peritonitis in the baboon: a primate model which stimulates human sepsis.
    Authors: Kinasewitz GT, 2016, Chang AC, Peer GT, Hinshaw LB, Taylor FB
    , 2000;13(2):100-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Type: Plasma
    Application: ELISA Development



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