抗CD80抗体(Anti-CD80, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80Extracellular domain
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human B7‑1/CD80 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for B7‑1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	B7‑1/CD80 in Human Tonsil. B7‑1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7‑1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

B7-1/CD80

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Background: B7-1/CD80 B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in thedown‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through IFN-gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.

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Citations

1. Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma
   Authors: P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang
   Oncol Lett, 2017;14(2):2458-2462.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


2. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
   Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
   Am. J. Pathol., 2011;178(2):744-53.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


3. Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis.
   Authors: Garin EH, Mu W, Arthur JM
   Kidney Int., 2010;78(3):296-302.
   Species: Human
   Sample Type: Urine
   Application: WB


4. HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis.
   Authors: Kuhne M, Erben U, Schulze-Tanzil G, Kohler D, Wu P, Richter FJ, John T, Radbruch A, Sieper J, Appel H
   Arthritis Rheum., 2009;60(6):1635-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
   Authors: Sakthivel P, Shively V, Kakoulidou M
   J. Intern. Med., 2007;261(4):399-407.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


6. Induction of B7-1 in podocytes is associated with nephrotic syndrome.
   Authors: Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P
   J. Clin. Invest., 2004;113(10):1390-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen

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