抗PD-1抗体(Anti-PD-1, Human, Goat-Poly antibody)
掲載日情報:2018/11/26 現在Webページ番号:26750
PD-1に対する抗体(Anti-PD-1, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年04月26日 20時55分現在]
詳細 | 商品名 |
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Anti-PD-1, Human, Goat-Poly |
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Anti-Human PD-1 Affinity Purified Polyclonal Ab |
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5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-PD-1, Human, Goat-Poly
文献数: 33
- 商品コード:AF1086
- メーカー:RSD
- 包装:100μg
- 価格:¥106,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | レセプター・リガンド相互作用ブロッキング用。マッチドペア:Human PD-1 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF1086,スタンダードとして#1086-PD-050を用いる。 別名:CD279 Genbank No: 5133 Protein Accession No: Q8IX89 |
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別包装品 | 別包装品あり | ||||||
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保存条件 | -20℃ | 法規備考 | |||||
抗原種 | Human | 免疫動物 | Goat | ||||
交差性 | Human | 適用 | Blocking,Western Blot,FCM,IHC,ELISA | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Human PD-1 Affinity Purified Polyclonal Ab
文献数: 5
- 商品コード:AF1086-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:CD279 Genbank No: 5133 Protein Accession No: Q8IX89 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Goat | |||||
交差性 | Human | 適用 | Blocking,Western Blot,FCM,IHC,ELISA | ||||
標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Polyclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Human |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human PD-1Leu25-Gln167Accession # Q8IX89 |
Source | Polyclonal Goat IgG |
Purification | Antigen Affinity-purified |
Specificity | Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 0.5-2 µg/mL | See below |
Flow Cytometry | 0.25 µg/106 cells | See below |
Immunohistochemistry | 5-15 µg/mL | See below |
Blockade of Receptor-ligand Interaction | In a functional ELISA, 3-12 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H1 Fc Chimera (Catalog # 156-B7) to immobilized Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding. | |
CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
Human PD-1 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human PD‑1 Antibody (Catalog #AF1086 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human PD‑1 Biotinylated Antibody (Catalog #BAF1086 ) |
ELISA Standard | Recombinant Human PD-1 Fc Chimera Protein, CF (Catalog #1086-PD ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Western Blot | ![]() click image to view larger | Detection of Human PD‑1 by Western Blot.Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD‑1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
Western Blot | ![]() click image to view larger | Detection of Human PD‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD‑1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
Flow Cytometry | ![]() click image to view larger | Detection of PD‑1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 μg/mL PHA overnight were stained with Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins. |
Immunohistochemistry | ![]() click image to view larger | PD‑1 in Human Lymph Node.PD‑1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
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