TGF-beta 1 Protein, CF 組換え体(リコンビナント)タンパク質 | Recombinant Human TGF-beta 1 Protein, CF
掲載日情報：2018/02/07 現在Webページ番号：215986
R&D Systems社製の高品質なTGF-beta 1 Protein, CFの組換え体タンパク質(Recombinant Human TGF-beta 1 Protein, CF)です。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Recombinant Human TGF-beta 1 Protein, CF
文献数： 0
- 商品コード：240-B-500/CF
- メーカー：RSD
- 包装：500μg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
純度：>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.，由来動物：Human，M.W.：12.8 kDa (monomer)，Genbank：7040，エンドトキシンレベル：<0.10 EU per 1 µg of the protein by the LAL method.，産生：CHO 別名：CEDLAP Genbank No： 7040 Protein Accession No： P01137 |
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| 保存条件 | -20℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） Helper T Cells Marker Antibodies |
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| 関連記事 | |||
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CF(Carrier-Free)とは？
R&D Systems社組換え体タンパク質製品では通常ウシ血清アルブミン(BSA)をキャリアタンパク質として加えています。キャリアタンパク質を加えることで組換え体タンパク質の安定性が高まり、使用期限が長くなります。また、より濃度の低い溶液での保管も可能となります。CF製品はBSAが含まれない製品となります。一般的に細胞培養や、ELISAのスタンダードにはBSA含有製品を推奨しています。CF製品はBSAが影響してしまうアプリケーションに推奨されます。
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Product Details
- Source
- Chinese Hamster Ovary cell line, CHO-derivedAla279-Ser390
- Accession #
- P01137
- Purity
- >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Predicted Molecular Mass
- 12.8 kDa (monomer)
- SDS-PAGE
- 12 kDa, reducing conditions24 kDa, non-reducing conditions
- Activity
- Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.The specific activity of Recombinant Human TGF-beta 1 is approximately 2.5 x 104 U/μg, which is calibrated against human TGF‑ beta 1 Standard (NIBSC code: 89/514).
- Bioactivity
- Recombinant Human TGF-beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
- SDS-PAGE
- 1 μg/lane of Recombinant Human TGF-beta 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
- Bioactivity
- Epithelial to Mesenchymal Transition (EMT) was induced in the A549 human lung carcinoma cell line with cell culture media supplemented with Recombinant Human (rh) TGF-beta 1 (Catalog # 240-B). Control cells were cultured without rhTGF-beta 1. EMT induction was confirmed at 48 h by flow cytometric staining for E-Cadherin (filled; Catalog # FAB18381P), an epithelial cell marker, or an isotype control (Catalog # IC0041P). TGF-beta 1 decreased the expression of E-Cadherin.
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Related Product & Information
- Entrez Gene IDs
- 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
- Background
- TGF-beta 1
- TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF-beta isoform has some non‑redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF‑ beta 1 shares 100% aa identity with pig, dog and cow TGF‑ beta 1, and 99% aa identity with mouse, rat and horse TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Fibroblast growth factor 2 (FGF2) regulates cytoglobin expression and activation of human hepatic stellate cells via JNK signaling
Authors: M Sato-Matsu, T Matsubara, A Daikoku, Y Okina, L Longato, K Rombouts, LTT Thuy, J Adachi, T Tomonaga, K Ikeda, K Yoshizato, M Pinzani, N Kawada
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: M Sato-Matsu, T Matsubara, A Daikoku, Y Okina, L Longato, K Rombouts, LTT Thuy, J Adachi, T Tomonaga, K Ikeda, K Yoshizato, M Pinzani, N Kawada
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Activation-induced cytidine deaminase targets SUV4-20-mediated histone H4K20 trimethylation to class-switch recombination sites
Authors: VC Rodríguez-, P Martínez-R, F Català-Mol, J Rodríguez-, A Garcia-Gom, G Poorani-Su, L Ciudad, H Hernando, A Pérez-Garc, C Company, JM Urquiza, AR Ramiro, JM Di Noia, A Vaquero, E Ballestar
Sci Rep, 2017;7(1):7594.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Authors: VC Rodríguez-, P Martínez-R, F Català-Mol, J Rodríguez-, A Garcia-Gom, G Poorani-Su, L Ciudad, H Hernando, A Pérez-Garc, C Company, JM Urquiza, AR Ramiro, JM Di Noia, A Vaquero, E Ballestar
Sci Rep, 2017;7(1):7594.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Fibronectin-guided migration of carcinoma collectives
Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg
Nat Commun, 2017;8(0):14105.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: S Gopal, L Veracini, D Grall, C Butori, S Schaub, S Audebert, L Camoin, E Baudelet, A Radwanska, S Beghelli-d, SM Violette, PH Weinreb, S Rekima, M Ilie, A Sudaka, P Hofman, E Van Obberg
Nat Commun, 2017;8(0):14105.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
A ZIP6-ZIP10 heteromer controls NCAM1 phosphorylation and integration into focal adhesion complexes during epithelial-to-mesenchymal transition
Authors: D Brethour, M Mehrabian, D Williams, X Wang, F Ghodrati, S Ehsani, EA Rubie, JR Woodgett, J Sevalle, Z Xi, E Rogaeva, G Schmitt-Ul
Sci Rep, 2017;7(0):40313.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: D Brethour, M Mehrabian, D Williams, X Wang, F Ghodrati, S Ehsani, EA Rubie, JR Woodgett, J Sevalle, Z Xi, E Rogaeva, G Schmitt-Ul
Sci Rep, 2017;7(0):40313.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis
Authors: MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat
Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Authors: MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat
Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Armc5 deletion causes developmental defects and compromises T-cell immune responses
Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
Nat Commun, 2017;8(0):13834.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
Nat Commun, 2017;8(0):13834.
Species: Mouse
Sample Type: Whole Cells
Application: Bioassay
Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients
Authors: V Ruiz-Carpi, P Sandoval, A Aguilera, P Albar-Vizc, ML Perez-Loza, GT González-M, A Acuña-Ruiz, J García-Can, P Botías, MA Bajo, R Selgas, JA Sánchez-To, J Passlick-D, D Piecha, J Büchel, S Steppan, M López-Cabr
Sci Rep, 2017;7(0):44941.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: V Ruiz-Carpi, P Sandoval, A Aguilera, P Albar-Vizc, ML Perez-Loza, GT González-M, A Acuña-Ruiz, J García-Can, P Botías, MA Bajo, R Selgas, JA Sánchez-To, J Passlick-D, D Piecha, J Büchel, S Steppan, M López-Cabr
Sci Rep, 2017;7(0):44941.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Podocalyxin influences malignant potential by controlling epithelial-mesenchymal transition in lung adenocarcinoma
Authors: H Kusumoto, Y Shintani, R Kanzaki, T Kawamura, S Funaki, M Minami, I Nagatomo, E Morii, M Okumura
Cancer Sci., 2017;108(3):528-535.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: H Kusumoto, Y Shintani, R Kanzaki, T Kawamura, S Funaki, M Minami, I Nagatomo, E Morii, M Okumura
Cancer Sci., 2017;108(3):528-535.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
Authors: KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa
Oncogene, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa
Oncogene, 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
TGF beta-1 induced CD44v6-NOX4 signaling in pathogenesis of idiopathic pulmonary fibrosis
Authors: S Ghatak, VC Hascall, RR Markwald, C Feghali-Bo, CM Artlett, M Gooz, GS Bogatkevic, I Atanelishv, RM Silver, J Wood, VJ Thannickal, S Misra
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: S Ghatak, VC Hascall, RR Markwald, C Feghali-Bo, CM Artlett, M Gooz, GS Bogatkevic, I Atanelishv, RM Silver, J Wood, VJ Thannickal, S Misra
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Cytidine monophosphate kinase is inhibited by the TGF-? signalling pathway through the upregulation of miR-130b-3p in human epithelial ovarian cancer
Authors: D Zhou, L Zhang, W Sun, W Guan, Q Lin, W Ren, J Zhang, G Xu
Cell. Signal., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
Authors: D Zhou, L Zhang, W Sun, W Guan, Q Lin, W Ren, J Zhang, G Xu
Cell. Signal., 2017;0(0):.
Species: Human
Sample Type: Whole Cells
Application: Bioassay
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