抗Human IL-1 beta/IL-1F2抗体(Anti-Human IL-1 beta/IL-1F2 antibody)
掲載日情報：2021/01/28 現在Webページ番号：213938
Human IL-1 beta/IL-1F2に対する抗体(Anti-Human IL-1 beta/IL-1F2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
カートに商品を
追加しました。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R) |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R) |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R) |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R) |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月26日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R)
文献数： 0
- 商品コード：MAB601R-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。 別名：catabolin クローン：2805R Genbank No： 3553 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,Neutralising,Simple Western,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 使いっきり抗体 |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R)
文献数： 0
- 商品コード：MAB601R-500
- メーカー：RSD
- 包装：500μg
- 価格：¥245,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：catabolin クローン：2805R Genbank No： 3553 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,Neutralising,Simple Western,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R)
文献数： 0
- 商品コード：MAB601R-100
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：catabolin クローン：2805R Genbank No： 3553 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,Neutralising,Simple Western,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
Anti-Human IL-1 beta/IL-1F2 MAb (Clone 2805R)
文献数： 0
- 商品コード：MAB601R-01M
- メーカー：RSD
- 包装：1mg
- 価格：ご照会ください
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：catabolin クローン：2805R Genbank No： 3553 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,Neutralising,Simple Western,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Recombinant | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
カートに商品を
追加しました。
追加しました。
Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human IL-1 beta /IL-1F2 |
| Source | Recombinant Monoclonal Mouse IgG1 Clone # 2805R |
| Purification | Protein A or G purified from cell culture supernatant |
| Specificity | Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed. |
カートに商品を
追加しました。
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Simple Western | 10 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Human IL-1 beta /IL-1F2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human IL‑1 beta /IL‑1F2 Antibody (Catalog #MAB601R ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF201 ) |
| ELISA Standard | Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog #201-LB ) | |
| Neutralization | Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 50-200 ng/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
カートに商品を
追加しました。
追加しました。
Data Examples
| Western Blot |
![Western Blot IL-1 beta/IL-1F2 Antibody (2805R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_MAB601R_Western_Blot_20895.jpg) click image to view larger | Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 μg/mL LPS for 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry |
![Immunocytochemistry IL-1 beta/IL-1F2 Antibody (2805R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_MAB601R_Immunocytochemistry__Immunofluorescence_20887.jpg) click image to view larger | IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL LPS and 3 μM monensin for 24 hours using Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Simple Western |
![Simple Western IL-1 beta/IL-1F2 Antibody (2805R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_MAB601R_Simple_Western_20896.jpg) click image to view larger | Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 200 nM and 10 μg/mL LPS for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Neutralization |
![Neutralization IL-1 beta/IL-1F2 Antibody (2805R) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_MAB601R_Block_Neutralize_20872.jpg) click image to view larger | Cell Proliferation Induced by IL-1 beta /IL-1F2 and Neutralization by Human IL-1 beta /IL-1F2 Antibody. Recombinant HumanIL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601R). The ND50 is typically 50-200 ng/mL. |
| Reconstitution | Reconstitute at 0.5mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-1 beta/IL-1F2 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. |
|
カートに商品を
追加しました。
追加しました。
References
| Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. | |
| Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. | |
| Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. | |
| Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. | |
| March, C.J. et al. (1985) Nature 315:641. | |
| Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907. | |
| Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10. | |
| Long Name: | Interleukin 1 beta |
| Entrez Gene IDs: | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| Alternate Names: | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
カートに商品を
追加しました。
追加しました。
Related Research Areas
| Western Blot | |
|---|---|
 | Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 μg/mL LPS for 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL LPS and 3 μM monensin for 24 hours using Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Simple Western | |
 | Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 200 nM and 10 μg/mL LPS for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601R). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Neutralization | |
 | Cell Proliferation Induced by IL-1 beta /IL-1F2 and Neutralization by Human IL-1 beta /IL-1F2 Antibody. Recombinant HumanIL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601R). The ND50 is typically 50-200 ng/mL. |
カートに商品を
追加しました。
追加しました。
Related Product & Information
| Long Name | Interleukin 1 beta |
|---|---|
| Entrez Gene IDs | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| Background | IL-1 beta/IL-1F2 |
| background_content | Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. |
カートに商品を
追加しました。
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。