Mouse CCL21/6Ckine Quantikine ELISA Kit
掲載日情報：2018/02/01 現在Webページ番号：206227
R&D Systems製のMouse CCL21/6Ckineを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月28日 16時55分現在]
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[在庫・価格 ：2025年04月28日 16時55分現在]
Mouse CCL21/6Ckine Quantikine ELISA Kit
文献数： 0
- 商品コード：M6C00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：7.81-500 pg/mL，感度：0.489 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：CCL21, 6Ckine 別名：6Ckine Genbank No： 6366 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Mouse CCL21/6Ckine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
- Sensitivity
- 0.489 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
- Specificity
- Natural and recombinant mouse 6Ckine.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse CCL21/6Ckine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse 6Ckine in tissue culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse 6Ckine and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse 6Ckine. Results obtained using natural mouse 6Ckine showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse 6Ckine.
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Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Recovery
The recovery of mouse 6Ckine spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 88-104 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse 6Ckine were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Entrez Gene IDs
- 6366 (Human); 18829 (Mouse); 298006 (Rat)
- Background
- CCL21/6Ckine
- CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a homeostatic chemokine that signals through CCR7 or CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) where it promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. Its upregulation during chronic inflammation or tissue damage promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients.
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